ArticleMolecular biology of the cell2007
Villin severing activity enhances actin-based motility in vivo.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- A first-in-class small-molecule inhibitor targeting AVIL exhibits safety and antitumor efficacy in preclinical models of glioblastoma.Science translational medicine · 2026Article
- Article
- Bioinformatics analyses of the proteome ofFrontiers in immunology · 2025Article
- Villin is a biomarker for reverse polarity in colorectal micropapillary carcinoma.Oncology letters · 2021Article
- Actin Assembly around the Shigella-Containing Vacuole Promotes Successful Infection.Cell reports · 2020Article
- Transcytosis subversion by M cell-to-enterocyte spread promotes Shigella flexneri and Listeria monocytogenes intracellular bacterial dissemination.PLoS pathogens · 2020Article
- Charge-dependent interactions of monomeric and filamentous actin with lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Building and repairing the stereocilia cytoskeleton in mammalian auditory hair cells.Hearing research · 2019Review
- CIB2 interacts with TMC1 and TMC2 and is essential for mechanotransduction in auditory hair cells.Nature communications · 2017Article
- Article
- By moonlighting in the nucleus, villin regulates epithelial plasticity.Molecular biology of the cell · 2016Article
- Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton.Cell host & microbe · 2015Article
- Physical model for the geometry of actin-based cellular protrusions.Biophysical journal · 2014Article
- Enterocyte loss of polarity and gut wound healing rely upon the F-actin-severing function of villin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Regulation of directional cell migration by membrane-induced actin bundling.Journal of cell science · 2013Article
- Pathogens and polymers: microbe-host interactions illuminate the cytoskeleton.The Journal of cell biology · 2011Review
- Arabidopsis VILLIN5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth.The Plant cell · 2010Article
- Calcium-actin waves and oscillations of cellular membranes.Biophysical journal · 2009Article
- Regulation of cell structure and function by actin-binding proteins: villin's perspective.FEBS letters · 2008Review
- New insights into mechanism and regulation of actin capping protein.International review of cell and molecular biology · 2008Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Villin, an actin-binding protein associated with the actin bundles that support microvilli, bundles, caps, nucleates, and severs actin in a calcium-dependant manner in vitro. We hypothesized that the severing activity of villin is responsible for its reported role in enhancing cell plasticity and motility. To test this hypothesis, we chose a loss of function strategy and introduced mutations in villin based on sequence comparison with CapG. By pyrene-actin assays, we demonstrate that this mutant has a strongly reduced severing activity, whereas nucleation and capping remain unaffected. The bundling activity and the morphogenic effects of villin in cells are also preserved in this mutant. We thus succeeded in dissociating the severing from the three other activities of villin. The contribution of villin severing to actin dynamics is analyzed in vivo through the actin-based movement of the intracellular bacteria Shigella flexneri in cells expressing villin and its severing variant. The severing mutations abolish the gain of velocity induced by villin. To further analyze this effect, we reconstituted an in vitro actin-based bead movement in which the usual capping protein is replaced by either the wild type or the severing mutant of villin. Confirming the in vivo results, villin-severing activity enhances the velocity of beads by more than two-fold and reduces the density of actin in the comets. We propose a model in which, by severing actin filaments and capping their barbed ends, villin increases the concentration of actin monomers available for polymerization, a mechanism that might be paralleled in vivo when an enterocyte undergoes an epithelio-mesenchymal transition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.