ArticleMolecular biology of the cell2007
The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivo.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis.Nature structural & molecular biology · 2026Article
- Structure, function and assembly of nuclear pore complexes.Nature reviews. Molecular cell biology · 2026Review
- Dynamic molecular mechanism of the nuclear pore complex permeability barrier.bioRxiv : the preprint server for biology · 2023Article
- Review
- Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.Cold Spring Harbor perspectives in biology · 2022Review
- Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress.The Journal of cell biology · 2020Article
- Allosteric modulation of nucleoporin assemblies by intrinsically disordered regions.Science advances · 2019Article
- Fantastic nuclear envelope herniations and where to find them.Biochemical Society transactions · 2018Review
- Recent advances in understanding nuclear size and shape.Nucleus (Austin, Tex.) · 2016Review
- The nuclear pore complex--structure and function at a glance.Journal of cell science · 2015Review
- Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration.Molecular biology of the cell · 2014Article
- Trafficking to uncharted territory of the nuclear envelope.Current opinion in cell biology · 2012Review
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- Time-course proteome analysis reveals the dynamic response of Cryptococcus gattii cells to fluconazole.PloS one · 2012Article
- Importazole, a small molecule inhibitor of the transport receptor importin-β.ACS chemical biology · 2011Article
- Lumenal interactions in nuclear pore complex assembly and stability.Molecular biology of the cell · 2011Article
- Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells.Chromosoma · 2010Article
- Nuclear pore biogenesis into an intact nuclear envelope.Chromosoma · 2010Review
- Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution.The Journal of cell biology · 2010Article
- Yeast Mpk1 cell wall integrity mitogen-activated protein kinase regulates nucleocytoplasmic shuttling of the Swi6 transcriptional regulator.Molecular biology of the cell · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nuclear pore complex (NPC) assembly in interphase cells requires that new NPCs insert into an intact nuclear envelope (NE). Our previous work identified the Ran GTPase as an essential component in this process. We proposed that Ran is required for targeting assembly factors to the cytoplasmic NE face via a novel, vesicular intermediate. Although the molecular target was not identified, Ran is known to function by modulating protein interactions for karyopherin (Kap) beta family members. Here we characterize loss-of-function Saccharomyces cerevisiae mutants in KAP95 with blocks in NPC assembly. Similar to defects in Ran cycle mutants, nuclear pore proteins are no longer localized properly to the NE in kap95 mutants. Also like Ran cycle mutants, the kap95-E126K mutant displayed enhanced lethality with nic96 and nup170 mutants. Thus, Kap95 and Ran are likely functioning at the same stage in assembly. However, although Ran cycle mutants accumulate small cytoplasmic vesicles, cells depleted of Kap95 accumulated long stretches of cytoplasmic membranes and had highly distorted NEs. We conclude that Kap95 serves as a key regulator of NPC assembly into intact NEs. Furthermore, both Kap95 and Ran may provide spatial cues necessary for targeting of vesicular intermediates in de novo NPC assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.