ArticleMolecular biology of the cell2007
Direct binding to Rsp5 mediates ubiquitin-independent sorting of Sna3 via the multivesicular body pathway.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 76 citations in OpenAlex.
- Rethinking the Yeast Endomembrane System.Sub-cellular biochemistry · 2026Review
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- Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of Abscisic Acid Receptors.The Plant cell · 2019Article
- Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI.Traffic (Copenhagen, Denmark) · 2018Article
- Resistance to the Plant Defensin NaD1 Features Modifications to the Cell Wall and Osmo-Regulation Pathways of Yeast.Frontiers in microbiology · 2018Article
- Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi.PloS one · 2018Article
- Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin hydrolase.Molecular biology of the cell · 2017Article
- Structural insights into the catalysis and regulation of E3 ubiquitin ligases.Nature reviews. Molecular cell biology · 2016Review
- Electron microscopy for ultrastructural analysis and protein localization inMicrobial cell (Graz, Austria) · 2015Review
- ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis.The Plant cell · 2015Article
- Influence of cellular trafficking pathway on bluetongue virus infection in ovine cells.Viruses · 2015Article
- A family of tetraspans organizes cargo for sorting into multivesicular bodies.Developmental cell · 2015Article
- The Legionella pneumophila effector protein, LegC7, alters yeast endosomal trafficking.PloS one · 2015Article
- Review
- Protein homeostasis at the plasma membrane.Physiology (Bethesda, Md.) · 2014Review
- Article
- Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast.The Journal of biological chemistry · 2013Article
- Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III.Journal of cell science · 2013Article
- Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3.Traffic (Copenhagen, Denmark) · 2012Article
- ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting.The Journal of cell biology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The sorting of most integral membrane proteins into the lumenal vesicles of multivesicular bodies (MVBs) is dependent on the attachment of ubiquitin (Ub) to their cytosolic domains. However, Ub is not required for sorting of Sna3, an MVB vesicle cargo protein in yeast. We show that Sna3 circumvents Ub-mediated recognition by interacting directly with Rsp5, an E3 Ub ligase that catalyzes monoubiquitination of MVB vesicle cargoes. The PPAY motif in the C-terminal cytosolic domain of Sna3 binds the WW domains in Rsp5, and Sna3 is polyubiquitinated as a consequence of this association. However, Ub does not appear to be required for transport of Sna3 via the MVB pathway because its sorting occurs under conditions in which its ubiquitination is impaired. Consistent with Ub-independent function of the MVB pathway, we show by electron microscopy that the formation of MVB vesicles does not require Rsp5 E3 ligase activity. However, cells expressing a catalytically disabled form of Rsp5 have a greater frequency of smaller MVB vesicles compared with the relatively broad distribution of vesicles seen in MVBs of wild-type cells, suggesting that the formation of MVB vesicles is influenced by Rsp5-mediated ubiquitination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.