ArticleMolecular biology of the cell2007
Characterization of multiple multivesicular body sorting determinants within Sna3: a role for the ubiquitin ligase Rsp5.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer.Cell chemical biology · 2021Article
- Protein Quality Control at the Mitochondrial Surface.Frontiers in cell and developmental biology · 2021Review
- Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases.Traffic (Copenhagen, Denmark) · 2017Article
- Deubiquitinating enzymes Ubp2 and Ubp15 regulate endocytosis by limiting ubiquitination and degradation of ARTs.Molecular biology of the cell · 2017Article
- Structural insights into the catalysis and regulation of E3 ubiquitin ligases.Nature reviews. Molecular cell biology · 2016Review
- Influence of cellular trafficking pathway on bluetongue virus infection in ovine cells.Viruses · 2015Article
- A family of tetraspans organizes cargo for sorting into multivesicular bodies.Developmental cell · 2015Article
- Tetraspan cargo adaptors usher GPI-anchored proteins into multivesicular bodies.Cell cycle (Georgetown, Tex.) · 2015Article
- Protein homeostasis at the plasma membrane.Physiology (Bethesda, Md.) · 2014Review
- Article
- Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast.The Journal of biological chemistry · 2013Article
- Sorting of the yeast vacuolar-type, proton-translocating ATPase enzyme complex (V-ATPase): identification of a necessary and sufficient Golgi/endosomal retention signal in Stv1p.The Journal of biological chemistry · 2012Article
- ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting.The Journal of cell biology · 2012Article
- Sna3 is an Rsp5 adaptor protein that relies on ubiquitination for its MVB sorting.Traffic (Copenhagen, Denmark) · 2012Article
- Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria.The EMBO journal · 2012Article
- Potential contribution of exosomes to the prion-like propagation of lesions in Alzheimer's disease.Frontiers in physiology · 2012Article
- ESCRT-independent budding of HIV-1 gag virus-like particles from Saccharomyces cerevisiae spheroplasts.PloS one · 2012Article
- How ubiquitin functions with ESCRTs.Traffic (Copenhagen, Denmark) · 2011Review
- Structure and function of a HECT domain ubiquitin-binding site.EMBO reports · 2011Article
- The ESCRT complexes.Critical reviews in biochemistry and molecular biology · 2010Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
A subset of proteins that transit the endosomal system are directed into the intralumenal vesicles of multivesicular bodies (MVBs). MVB formation is critical for a variety of cellular functions including receptor down-regulation, viral budding, antigen presentation, and the generation of lysosome-related organelles. Entry of transmembrane proteins into the intralumenal vesicles of a MVB is a highly regulated process that is positively modulated by covalent modification of cargoes with ubiquitin. To identify additional MVB sorting signals, we examined the previously described ubiquitination-independent MVB cargo Sna3. Although Sna3 ubiquitination is not essential, Sna3 MVB sorting is positively modulated by its ubiquitination. Examination of MVB sorting determinants within a form of Sna3 lacking all lysine residues identified two critical regions: an amino-terminal tyrosine-containing region and a carboxyl-terminal PPAY motif. This PPAY motif interacts with the WW domains of the ubiquitin ligase Rsp5, and mutations in either the WW or, surprisingly, the HECT domains of Rsp5 negatively impacted MVB targeting of lysine-minus Sna3. These data indicate that Rsp5 function is required for MVB targeting of Sna3 in a capacity beyond cargo ubiquitination. These results uncover a series of determinants impacting Sna3 MVB sorting, including unexpected roles for Rsp5.
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