ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2006
Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- Linking GWAS risk genes to transcriptional features of major depressive disorder via in vivo Perturb-seq.Nature genetics · 2026Article
- DENND1A desensitizes granulosa cells to FSH by arresting intracellular FSHR transportation.Science China. Life sciences · 2024Article
- Synaptic Vesicle Recycling and the Endolysosomal System: A Reappraisal of Form and Function.Frontiers in synaptic neuroscience · 2022Review
- A novel DENND1B-localized structure found at the basal side of adherent cells.Histochemistry and cell biology · 2021Article
- Suppression of Tumorigenicity 5 Ameliorates Tumor Characteristics of Invasive Breast Cancer CellsFrontiers in oncology · 2021Article
- Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.The Journal of biological chemistry · 2020Article
- Association analysis between the tag single nucleotide polymorphisms of DENND1A and the risk of polycystic ovary syndrome in Chinese Han women.BMC medical genetics · 2020Article
- Intersectin-s interaction with DENND2B facilitates recycling of epidermal growth factor receptor.EMBO reports · 2017Article
- SCD1 and SCD2 Form a Complex That Functions with the Exocyst and RabE1 in Exocytosis and Cytokinesis.The Plant cell · 2017Article
- Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Molecular Machines Determining the Fate of Endocytosed Synaptic Vesicles in Nerve Terminals.Frontiers in synaptic neuroscience · 2016Review
- Phosphorylation-dependent Regulation of Connecdenn/DENND1 Guanine Nucleotide Exchange Factors.The Journal of biological chemistry · 2015Article
- Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis.PLoS biology · 2013Article
- Mediation of clathrin-dependent trafficking during cytokinesis and cell expansion by Arabidopsis stomatal cytokinesis defective proteins.The Plant cell · 2013Article
- RabGEFs are a major determinant for specific Rab membrane targeting.The Journal of cell biology · 2013Article
- Genetic alterations within the DENND1A gene in patients with polycystic ovary syndrome (PCOS).PloS one · 2013Article
- Reversible phosphocholination of Rab proteins by Legionella pneumophila effector proteins.The EMBO journal · 2012Article
- Replication of association of DENND1A and THADA variants with polycystic ovary syndrome in European cohorts.Journal of medical genetics · 2012Article
- Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clathrin-coated vesicles (CCVs) are responsible for the endocytosis of multiple cargo, including synaptic vesicle membranes. We now describe a new CCV protein, termed connecdenn, that contains an N-terminal DENN (differentially expressed in neoplastic versus normal cells) domain, a poorly characterized protein module found in multiple proteins of unrelated function and a C-terminal peptide motif domain harboring three distinct motifs for binding the alpha-ear of the clathrin adaptor protein 2 (AP-2). Connecdenn coimmunoprecipitates and partially colocalizes with AP-2, and nuclear magnetic resonance and peptide competition studies reveal that all three alpha-ear-binding motifs contribute to AP-2 interactions. In addition, connecdenn contains multiple Src homology 3 (SH3) domain-binding motifs and coimmunoprecipitates with the synaptic SH3 domain proteins intersectin and endophilin A1. Interestingly, connecdenn is enriched on neuronal CCVs and is present in the presynaptic compartment of neurons. Moreover, connecdenn has a uniquely stable association with CCV membranes because it resists extraction with Tris and high-salt buffers, unlike most other CCV proteins, but it is not detected on purified synaptic vesicles. Together, these observations suggest that connecdenn functions on the endocytic limb of the synaptic vesicle cycle. Accordingly, disruption of connecdenn interactions with its binding partners through overexpression of the C-terminal peptide motif domain or knock down of connecdenn through lentiviral delivery of small hairpin RNA both lead to defects in synaptic vesicle endocytosis in cultured hippocampal neurons. Thus, we identified connecdenn as a component of the endocytic machinery functioning in synaptic vesicle endocytosis, providing the first evidence of a role for a DENN domain-containing protein in endocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.