ArticleJournal of clinical microbiology2007
A genotype-independent real-time PCR assay for quantification of hepatitis B virus DNA.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Integration of Viral Genome to Human Genomic DNA in Nails of Patients with Chronic Hepatitis B Virus Infection.JMA journal · 2023Article
- Viral Diagnosis of Hepatitis B and Delta: What We Know and What Is Still Required? Specific Focus on Low- and Middle-Income Countries.Microorganisms · 2022Review
- Novel Neplanocin A Derivatives as Selective Inhibitors of Hepatitis B Virus with a Unique Mechanism of Action.Antimicrobial agents and chemotherapy · 2022Article
- Signature of chronic hepatitis B virus infection in nails and hair.BMC infectious diseases · 2022Article
- Chronic hepatitis B in pregnant women: Current trends and approaches.World journal of gastroenterology · 2021Review
- Article
- Effect of viral DNA methylation on expression of hepatitis B virus proteins depends on the virus genotype.Virus genes · 2020Article
- Deep sequencing of hepatitis B surface antigen gene in the preserved umbilical cords in immunoprophylaxis failure against mother-to-child HBV transmission.BMC infectious diseases · 2019Article
- Differences in sequences between HBV-relaxed circular DNA and covalently closed circular DNA.Emerging microbes & infections · 2017Article
- Identification of hydrolyzable tannins (punicalagin, punicalin and geraniin) as novel inhibitors of hepatitis B virus covalently closed circular DNA.Antiviral research · 2016Article
- Evaluation of the G145R Mutant of the Hepatitis B Virus as a Minor Strain in Mother-to-Child Transmission.PloS one · 2016Article
- Identification of Antiviral Agents Targeting Hepatitis B Virus Promoter from Extracts of Indonesian Marine Organisms by a Novel Cell-Based Screening Assay.Marine drugs · 2015Article
- Lack of infectivity of HBV in feces from patients with chronic hepatitis B virus infection, and infection using chimeric mice.BMC research notes · 2015Article
- Recent advances in molecular diagnostics of hepatitis B virus.World journal of gastroenterology · 2014Review
- High diversity of hepatitis B virus genotypes in Panamanian blood donors: a molecular analysis of new variants.PloS one · 2014Article
- Interleukin-1 and tumor necrosis factor-α trigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID).The Journal of biological chemistry · 2013Article
- Molecular characterisation of hepatitis B virus in the resident Chinese population in Panama City.Memorias do Instituto Oswaldo Cruz · 2013Article
- Hepatitis B surface gene 145 mutant as a minor population in hepatitis B virus carriers.BMC research notes · 2012Article
- Development of real-time PCR array for simultaneous detection of eight human blood-borne viral pathogens.PloS one · 2012Article
- Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line.World journal of gastroenterology · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Accurate quantification of hepatitis B virus (HBV) DNA levels is important for monitoring patients with chronic HBV infection and for assessing their responses to antiviral therapy. This study aimed to develop a real-time PCR assay that is sensitive and can accurately quantify a wide range of HBV DNA levels across the known HBV genotypes. An "in-house" real-time PCR assay using primers and a TaqMan probe in a highly conserved region of the HBV surface gene was designed. The assay was standardized against a WHO standard and validated against plasmids of HBV genotypes A through H. The linear quantification range was approximately 5 x 10(0) to 2.0 x 10(9) IU/ml. Results of samples from patients infected with HBV genotypes A through H tested using our real-time "in-house" PCR assay showed an excellent correlation with those of the Cobas Amplicor HBV Monitor (R2=0.9435) and the Cobas TaqMan HBV (R2=0.9873) tests. We have established a real-time PCR assay that is genotype independent and can accurately quantify a wide range of HBV DNA levels. Further studies of additional samples are ongoing to validate the genotype independence of our assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.