ArticleJournal of clinical microbiology2007
Vibrio cholerae strain typing and phylogeny study based on simple sequence repeats.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 81 citations in OpenAlex.
- Molecular Variation of Methicillin-resistantRecent patents on biotechnology · 2026Article
- Multidrug-resistant clinical K. pneumoniae ST16, ST218, and ST283 and emergence of pandrug-resistant KPC-positive ST6434/K2 lineage in Iraq.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Molecular characterization ofJournal of medical microbiology · 2021Article
- A Vibrio cholerae Core Genome Multilocus Sequence Typing Scheme To Facilitate the Epidemiological Study of Cholera.Journal of bacteriology · 2020Article
- A molecular and epidemiological study of Vibrio cholerae isolates from cholera outbreaks in southern Ghana.PloS one · 2020Article
- Vibrio cholerae autoinducer-1 enhances the virulence of enteropathogenic Escherichia coli.Scientific reports · 2019Article
- A prospective cohort study comparing household contact and water Vibrio cholerae isolates in households of cholera patients in rural Bangladesh.PLoS neglected tropical diseases · 2018Article
- Cholera Epidemic in South Sudan and Uganda and Need for International Collaboration in Cholera Control.Emerging infectious diseases · 2018Article
- Comparison of virulence gene profiles and genomic fingerprints ofTropical medicine and health · 2018Article
- Comparison and Evaluation of the Molecular Typing Methods for ToxigenicFrontiers in microbiology · 2018Article
- Genetic relatedness of Vibrio cholerae isolates within and between households during outbreaks in Dhaka, Bangladesh.BMC genomics · 2017Article
- Genome-Wide Biases in the Rate and Molecular Spectrum of Spontaneous Mutations in Vibrio cholerae and Vibrio fischeri.Molecular biology and evolution · 2017Article
- Comparison of inferred relatedness based on multilocus variable-number tandem-repeat analysis and whole genome sequencing of Vibrio cholerae O1.FEMS microbiology letters · 2016Article
- Molecular Epidemiology and Antibiotic Susceptibility of Vibrio cholerae Associated with a Large Cholera Outbreak in Ghana in 2014.PLoS neglected tropical diseases · 2016Article
- Evaluation in Cameroon of a Novel, Simplified Methodology to Assist Molecular Microbiological Analysis of V. cholerae in Resource-Limited Settings.PLoS neglected tropical diseases · 2016Article
- A metalloprotease secreted by the type II secretion system links Vibrio cholerae with collagen.Journal of bacteriology · 2015Article
- Molecular tools in understanding the evolution of Vibrio cholerae.Frontiers in microbiology · 2015Review
- Genetic variation of Vibrio cholerae during outbreaks, Bangladesh, 2010-2011.Emerging infectious diseases · 2014Article
- Improved short-sequence-repeat genotyping of Mycobacterium avium subsp. paratuberculosis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry.Applied and environmental microbiology · 2014Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio cholerae is the etiological agent of cholera. Its natural reservoir is the aquatic environment. To date, practical typing of V. cholerae is mainly serological and requires about 200 antisera. Simple sequence repeats (SSR), also termed VNTR (for variable number of tandem repeats), provide a source of high genomic polymorphism used in bacterial typing. Here we describe an SSR-based typing method that combines the variation in highly mutable SSR loci, with that of shorter, relatively more stable mononucleotide repeat (MNR) loci, for accurate and rapid typing of V. cholerae. In silico screening of the V. cholerae genome revealed thousands of perfect SSR tracts with an average frequency of one SSR every 152 bp. A panel of 32 V. cholerae strains, representing both clinical and environmental isolates, was tested for polymorphism in SSR loci. Two strategies were applied to identify SSR variation: polymorphism of SSR tracts longer than 12 bp (L-SSR) assessed by capillary fragment-size analysis and MNR polymorphism assessed by sequencing. The nine L-SSR loci tested were all polymorphic, displaying 2 to 13 alleles per locus. Sequence analysis of eight MNR-containing loci (MNR-multilocus sequence typing [MLST]) provided information on both variations in the MNR tract itself, and single nucleotide polymorphism (SNP) in their flanking sequences. Phylogenetic analysis of the combined SSR data showed a clear discrimination between the clinical strains belonging to O1 and O139 serogroups, and the environmental isolates. Furthermore, discrimination between 27 strains of the 32 strains was achieved. SSR-based typing methods combining L-SSR and MNR-MLST were found to be efficient for V. cholerae typing.
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