ArticleChromosoma2007
The cenH3 histone variant defines centromeres in Giardia intestinalis.
Article in Chromosoma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Cytotoxic effects of ivermectin onFrontiers in microbiology · 2024Article
- Analysis of the role of acetylation inFrontiers in microbiology · 2024Article
- Nucleosome Structures Built from Highly Divergent Histones: Parasites and Giant DNA Viruses.Epigenomes · 2022Review
- A polo-like kinase modulates cytokinesis and flagella biogenesis in Giardia lamblia.Parasites & vectors · 2021Article
- Evolution of metabolic capabilities and molecular features of diplonemids, kinetoplastids, and euglenids.BMC biology · 2020Article
- Article
- Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis.PLoS biology · 2019Article
- Simple and Complex Centromeric Satellites inGenetics · 2018Article
- Phylogenetic analysis of the core histone doublet and DNA topo II genes of Marseilleviridae: evidence of proto-eukaryotic provenance.Epigenetics & chromatin · 2017Article
- Evolutionary dynamics of the kinetochore network in eukaryotes as revealed by comparative genomics.EMBO reports · 2017Article
- Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.MicrobiologyOpen · 2016Article
- Evolutionary Turnover of Kinetochore Proteins: A Ship of Theseus?Trends in cell biology · 2016Review
- Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis.Chromosoma · 2015Article
- Recurrent loss of CenH3 is associated with independent transitions to holocentricity in insects.eLife · 2014Article
- Holocentric chromosomes: convergent evolution, meiotic adaptations, and genomic analysis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- A unified phylogeny-based nomenclature for histone variants.Epigenetics & chromatin · 2012Article
- Histone H3 Variants in Trichomonas vaginalis.Eukaryotic cell · 2012Article
- Dynamics of a novel centromeric histone variant CenH3 reveals the evolutionary ancestral timing of centromere biogenesis.Nucleic acids research · 2010Article
- Histone variants--ancient wrap artists of the epigenome.Nature reviews. Molecular cell biology · 2010Review
- Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spironucleus vortens.BMC microbiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Histone H3 variants play critical roles in the functional specialization of chromatin by epigenetically marking centromeric chromatin and transcriptionally active or silent genes. Specifically, the cenH3 histone variant acts as the primary epigenetic determinant of the site of kinetochore assembly at centromeres. Although the function of histone variants is well studied in plants, animals, and fungi, there is little knowledge of the evolutionary conservation of histone variants and their function in most protists. We find that Giardia intestinalis--a diplomonad parasite with two equivalent nuclei--has two phylogenetically distinct histone H3 variants with N-terminal extensions and nonconserved promoters. To determine their role in chromatin dynamics, conventional H3 and the two H3 variants were GFP-tagged, and their subcellular location was monitored during interphase and mitosis. We demonstrate that one cenH3-like variant has a conserved function in epigenetically marking centromeres. The other H3 variant (H3B) has a punctate distribution on chromosomes, but does not colocalize with active transcriptional regions as indicated by H3K4 methylation. We suggest that H3B could instead mark noncentromeric heterochromatin. Giardia is a member of the Diplomonads and represents an ancient divergence from metazoans and fungi. We confirm the ancient role of histone H3 variants in modulating chromatin architecture, and suggest that monocentric chromosomes represent an ancestral chromosome morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.