ArticleProceedings of the National Academy of Sciences of the United States of America1991
Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 200 citations in OpenAlex.
- Exploring cell cycle-mediated regulations of glycolysis in budding yeast.Frontiers in microbiology · 2023Article
- Cancer cell cycle dystopia: heterogeneity, plasticity, and therapy.Trends in cancer · 2022Review
- A human kinase yeast array for the identification of kinases modulating phosphorylation-dependent protein-protein interactions.Molecular systems biology · 2022Article
- Review
- Non-canonical approaches to targeting hypoxic tumors.American journal of cancer research · 2022Review
- Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2018Article
- Expression of CDK6 in Oral Squamous Cell CarcinomasAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Cdk5 activity in the brain - multiple paths of regulation.Journal of cell science · 2014Review
- The cloning of the cdk2 transcript and the localization of its expression during gametogenesis in the freshwater giant prawn, Macrobrachium rosenbergii.Molecular biology reports · 2013Article
- Functional cDNA expression cloning: pushing it to the limit.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2012Review
- Function following form: functional differentiation of mammary epithelial cells requires laminin-induced polarization of PI3-kinase.Cell cycle (Georgetown, Tex.) · 2011Article
- Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases.PLoS pathogens · 2010Article
- Putting one step before the other: distinct activation pathways for Cdk1 and Cdk2 bring order to the mammalian cell cycle.Cell cycle (Georgetown, Tex.) · 2010Article
- Paired-like homeodomain protein ESXR1 possesses a cleavable C-terminal region that inhibits cyclin degradation.Oncogene · 2004Article
- Regulation of MTK1/MEKK4 kinase activity by its N-terminal autoinhibitory domain and GADD45 binding.Molecular and cellular biology · 2002Article
- Common and reversible regulation of wild-type p53 function and of ribosomal biogenesis by protein kinases in human cells.Oncogene · 2001Article
- S and G2 phase roles for Cdk2 revealed by inducible expression of a dominant-negative mutant in human cells.Molecular and cellular biology · 2001Article
- RRS1, a conserved essential gene, encodes a novel regulatory protein required for ribosome biogenesis in Saccharomyces cerevisiae.Molecular and cellular biology · 2000Article
- Article
- Human and yeast cdk-activating kinases (CAKs) display distinct substrate specificities.Molecular biology of the cell · 1998Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We have cloned two different human cDNAs that can complement cdc28 mutations of budding yeast Saccharomyces cerevisiae. One corresponds to a gene encoding human p34CDC2 kinase, and the other to a gene (CDK2; cell division kinase) that has not been characterized previously. The CDK2 protein is highly homologous to p34CDC2 kinase (65% identical) and more significantly is homologous to Xenopus Eg1 kinase (89% identical), suggesting that CDK2 is the human homolog of Eg1. The human CDC2 and CDK2 genes were both able to complement the inviability of a null allele of S. cerevisiae CDC28. This result indicates that the CDK2 protein has a biological activity closely related to the CDC28 and p34CDC2 kinases. However, CDK2 was unable to complement cdc2 mutants in fission yeast Schizosaccharomyces pombe under the condition where the human CDC2 gene could complement them. CDK2 mRNA appeared late in G1 or in early S phase, slightly before CDC2 mRNA, after growth stimulation in normal human fibroblast cells. These results suggest that in human cells, two different CDC2-like kinases may regulate the cell cycle at distinct stages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.