ArticleNucleic acids research2007
The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Molecular models of bidirectional promoter regulation.Current opinion in structural biology · 2024Review
- Transcriptional directionality of the human insulin-degrading enzyme promoter.Molecular and cellular biochemistry · 2013Article
- Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.Nucleic acids research · 2011Article
- Role of an ER stress response element in regulating the bidirectional promoter of the mouse CRELD2 - ALG12 gene pair.BMC genomics · 2010Article
- High regulatability favors genetic selection in SLC18A2, a vesicular monoamine transporter essential for life.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Cooperation between NRF-2 and YY-1 transcription factors is essential for triggering the expression of the PREPL-C2ORF34 bidirectional gene pair.BMC molecular biology · 2009Article
- An imbalance in C/EBPs and increased mitochondrial activity in asthmatic airway smooth muscle cells: novel targets in asthma therapy?British journal of pharmacology · 2009Review
- Suppression mechanisms of COX assembly defects in yeast and human: insights into the COX assembly process.Biochimica et biophysica acta · 2009Review
- Roles of Oxa1-related inner-membrane translocases in assembly of respiratory chain complexes.Biochimica et biophysica acta · 2009Review
- Global analysis of in vivo Foxa2-binding sites in mouse adult liver using massively parallel sequencing.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genes for mitoribosomal protein S12 (Mrps12) and mitochondrial seryl-tRNA ligase (Sarsm and Sars2) are oppositely transcribed from a conserved promoter region of <200 bp in both human and mouse. Using a dual reporter vector we identified an array of 4 CCAAT box elements required for efficient transcription of the two genes in cultured mouse 3T3 cells, and for enforcing directionality in favour of Mrps12. Electrophoretic mobility shift assay (EMSA) and in vivo footprinting confirmed the importance of these promoter elements as sites of protein-binding, and EMSA supershift and chromatin immunoprecipitation (ChIP) assays identified NF-Y as the key transcription factor involved, revealing a common pattern of protein-DNA interactions in all tissues tested (liver, brain, heart, kidney and 3T3 cells). The inherently bidirectional activity of NF-Y makes it an especially suitable factor to govern promoters of this class, whose expression is linked to cell proliferation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.