ArticleThe Journal of biological chemistry2007
Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Structural insights into the binding of bS1 to the ribosome.Nucleic acids research · 2023Article
- Joachim Frank's Binding with the Ribosome.Structure (London, England : 1993) · 2019Review
- Dynamic analysis of ribosome by a movie made from many three-dimensional electron-microscopy density maps.Biophysics and physicobiology · 2019Article
- Rejection of tmRNA·SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA.RNA (New York, N.Y.) · 2014Article
- In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution.RNA (New York, N.Y.) · 2014Article
- Requirements for resuming translation in chimeric transfer-messenger RNAs of Escherichia coli and Mycobacterium tuberculosis.BMC molecular biology · 2014Article
- Mechanism of trans-translation revealed by in vitro studies.Frontiers in microbiology · 2014Review
- Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB.Frontiers in microbiology · 2014Review
- tmRNA-SmpB: a journey to the centre of the bacterial ribosome.The EMBO journal · 2010Article
- Role of the C-terminal tail of SmpB in the early stage of trans-translation.RNA (New York, N.Y.) · 2010Article
- Accommodation of tmRNA-SmpB into stalled ribosomes: a cryo-EM study.RNA (New York, N.Y.) · 2010Article
- Structural features of the tmRNA-ribosome interaction.RNA (New York, N.Y.) · 2009Article
- SmpB contributes to reading frame selection in the translation of transfer-messenger RNA.Journal of molecular biology · 2009Article
- Ribosome hijacking: a role for small protein B during trans-translation.EMBO reports · 2009Article
- The highest affinity binding site of small protein B on transfer messenger RNA is outside the tRNA domain.RNA (New York, N.Y.) · 2008Article
- The role of upstream sequences in selecting the reading frame on tmRNA.BMC biology · 2008Article
- Functional SmpB-ribosome interactions require tmRNA.The Journal of biological chemistry · 2007Article
- A functional interaction of SmpB with tmRNA for determination of the resuming point of trans-translation.RNA (New York, N.Y.) · 2007Article
- Interaction of SmpB with ribosome from directed hydroxyl radical probing.Nucleic acids research · 2007Article
- Ribosomal protein S1 influences trans-translation in vitro and in vivo.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
A eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer-messenger RNA, small protein B, and ribosome protein S1. By means of cryo-electron microscopy, we obtained a map of the complex composed of a stalled ribosome and small protein B, which appears near the decoding center. This result suggests that, when lacking a codon, the A-site on the small subunit is a target for small protein B. To investigate the role of S1 played in trans-translation, we obtained a cryo-electron microscopic map, including a stalled ribosome, transfer-messenger RNA, and small protein Bs but in the absence of S1. In this complex, several connections between the 30 S subunit and transfer-messenger RNA that appear in the +S1 complex are no longer found. We propose the unifying concept of scaffolding for the roles of small protein B and S1 in binding of transfer-messenger RNA to the ribosome during trans-translation, and we infer a pathway of sequential binding events in the initial phase of trans-translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.