ArticleGenetics2007
Exploring strategies for protein trapping in Drosophila.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
103 citing papers in PubMed, 165 citations in OpenAlex.
- HIF-1α-mediated feedback prevents TOR signalling from depleting oxygen supply and triggering stress during normal development.Nature communications · 2025Article
- dFlpTag, a RMCE-based tool for simultaneous endogenous protein tagging and cell labeling in Drosophila.Scientific reports · 2025Article
- Titin-dependent biomechanical feedback tailors sarcomeres to specialized muscle functions in insects.Science advances · 2025Article
- Wnt signaling couples G2 phase control with differentiation during hematopoiesis in Drosophila.Developmental cell · 2024Article
- The sex of organ geometry.Nature · 2024Article
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- Evidence that tissue recoil in the earlyMolecular biology of the cell · 2023Article
- Characterization of genetic and molecular tools for studying the endogenous expression ofbioRxiv : the preprint server for biology · 2023Article
- The Drosophila dopamine 2-like receptor D2R (Dop2R) is required in the blood brain barrier for male courtship.Genes, brain, and behavior · 2023Article
- Tagging Drosophila Proteins with Genetically Encoded Fluorophores.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Review
- Drosophila SERTAD domain protein Taranis is required in somatic cells for maintenance of male germline stem cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- GFP-Tagged Protein Detection by Electron Microscopy Using a GBP-APEX Tool inFrontiers in cell and developmental biology · 2021Article
- The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation inFrontiers in cellular neuroscience · 2021Article
- Article
- Condensin I is required for faithful meiosis in Drosophila males.Chromosoma · 2020Article
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Sex Differences in Intestinal Carbohydrate Metabolism Promote Food Intake and Sperm Maturation.Cell · 2019Article
- Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The use of fluorescent protein tags has had a huge impact on cell biological studies in virtually every experimental system. Incorporation of coding sequence for fluorescent proteins such as green fluorescent protein (GFP) into genes at their endogenous chromosomal position is especially useful for generating GFP-fusion proteins that provide accurate cellular and subcellular expression data. We tested modifications of a transposon-based protein trap screening procedure in Drosophila to optimize the rate of recovering useful protein traps and their analysis. Transposons carrying the GFP-coding sequence flanked by splice acceptor and donor sequences were mobilized, and new insertions that resulted in production of GFP were captured using an automated embryo sorter. Individual stocks were established, GFP expression was analyzed during oogenesis, and insertion sites were determined by sequencing genomic DNA flanking the insertions. The resulting collection includes lines with protein traps in which GFP was spliced into mRNAs and embedded within endogenous proteins or enhancer traps in which GFP expression depended on splicing into transposon-derived RNA. We report a total of 335 genes associated with protein or enhancer traps and a web-accessible database for viewing molecular information and expression data for these genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.