ArticleGenetics2007
Histone H3 lysine 36 methylation antagonizes silencing in Saccharomyces cerevisiae independently of the Rpd3S histone deacetylase complex.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Yeast epigenetics: the inheritance of histone modification states.Bioscience reports · 2019Review
- Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 inBiotechnology for biofuels · 2019Article
- Ten principles of heterochromatin formation and function.Nature reviews. Molecular cell biology · 2018Review
- The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.Molecular cell · 2017Article
- Article
- Article
- An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair.Nature communications · 2014Article
- The histone demethylase activity of Rph1 is not essential for its role in the transcriptional response to nutrient signaling.PloS one · 2014Article
- The many roles of the conserved eukaryotic Paf1 complex in regulating transcription, histone modifications, and disease states.Biochimica et biophysica acta · 2013Article
- Mechanism for epigenetic variegation of gene expression at yeast telomeric heterochromatin.Genes & development · 2012Article
- The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3.Molecular and cellular biology · 2011Article
- Rpd3-dependent boundary formation at telomeres by removal of Sir2 substrate.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Asf1 can promote trimethylation of H3 K36 by Set2.Molecular and cellular biology · 2010Article
- Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Article
- Set2-dependent K36 methylation is regulated by novel intratail interactions within H3.Molecular and cellular biology · 2009Article
- The ASH1 HOMOLOG 2 (ASHH2) histone H3 methyltransferase is required for ovule and anther development in Arabidopsis.PloS one · 2009Article
- DNA polymerase epsilon, acetylases and remodellers cooperate to form a specialized chromatin structure at a tRNA insulator.The EMBO journal · 2009Article
- Multiple histone modifications in euchromatin promote heterochromatin formation by redundant mechanisms in Saccharomyces cerevisiae.BMC molecular biology · 2009Article
- Mutational analysis of the Sir3 BAH domain reveals multiple points of interaction with nucleosomes.Molecular and cellular biology · 2009Article
- HistoneHits: a database for histone mutations and their phenotypes.Genome research · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In yeast, methylation of histone H3 on lysine 36 (H3-K36) is catalyzed by the NSD1 leukemia oncoprotein homolog Set2. The histone deacetylase complex Rpd3S is recruited to chromatin via binding of the chromodomain protein Eaf3 to methylated H3-K36 to prevent erroneous transcription initiation. Here we identify a distinct function for H3-K36 methylation. We used random mutagenesis of histones H3 and H4 followed by a reporter-based screen to identify residues necessary to prevent the ectopic spread of silencing from the silent mating-type locus HMRa into flanking euchromatin. Mutations in H3-K36 or deletion of SET2 caused ectopic silencing of a heterochromatin-adjacent reporter. Transcriptional profiling revealed that telomere-proximal genes are enriched for those that display decreased expression in a set2Delta strain. Deletion of SIR4 rescued the expression defect of 26 of 37 telomere-proximal genes with reduced expression in set2Delta cells, implying that H3-K36 methylation prevents the spread of telomeric silencing. Indeed, Sir3 spreads from heterochromatin into neighboring euchromatin in set2Delta cells. Furthermore, genetic experiments demonstrated that cells lacking the Rpd3S-specific subunits Eaf3 or Rco1 did not display the anti-silencing phenotype of mutations in SET2 or H3-K36. Thus, antagonism of silencing is independent of the only known effector of this conserved histone modification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.