ArticleThe Journal of experimental medicine2006
Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.
Article in The Journal of experimental medicine, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- The Humanization and Maturation of an Anti-PrPc Antibody.Bioengineering (Basel, Switzerland) · 2024Article
- Transcription factor binding at Ig enhancers is linked to somatic hypermutation targeting.European journal of immunology · 2020Article
- Enhancers Improve the AID-Induced Hypermutation in Episomal Vector for Antibody Affinity Maturation in Mammalian Cell Display.Antibodies (Basel, Switzerland) · 2018Article
- Review
- Characterisation of a stably integrated expression system for exogenous protein expression in DT40.Wellcome open research · 2017Article
- Bcl6 Is Required for Somatic Hypermutation and Gene Conversion in Chicken DT40 Cells.PloS one · 2016Article
- A critical context-dependent role for E boxes in the targeting of somatic hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- The IgH 3' regulatory region controls somatic hypermutation in germinal center B cells.The Journal of experimental medicine · 2013Article
- The role of activation-induced deaminase in antibody diversification and genomic instability.Immunologic research · 2013Review
- Harnessing gene conversion in chicken B cells to create a human antibody sequence repertoire.PloS one · 2013Article
- Regulation of AID, the B-cell genome mutator.Genes & development · 2013Review
- Identification of core DNA elements that target somatic hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Cellular origin and pathophysiology of chronic lymphocytic leukemia.The Journal of experimental medicine · 2012Article
- Negative supercoiling creates single-stranded patches of DNA that are substrates for AID-mediated mutagenesis.PLoS genetics · 2012Article
- Mismatch-mediated error prone repair at the immunoglobulin genes.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2011Review
- Targeting of AID-mediated sequence diversification to immunoglobulin genes.Current opinion in immunology · 2011Review
- Prevalent de novo somatic mutations in superantigen genes of mouse mammary tumor viruses in the genome of C57BL/6J mice and its potential implication in the immune system.BMC immunology · 2011Article
- Comparison of identical and functional Igh alleles reveals a nonessential role for Eμ in somatic hypermutation and class-switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Attracting AID to targets of somatic hypermutation.The Journal of experimental medicine · 2010Article
- AID and somatic hypermutation.Advances in immunology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It is thought that gene conversion (GCV) and somatic hypermutation (SHM) of immunoglobulin (Ig) genes occur in two steps: the generation of uracils in DNA by activation-induced cytidine deaminase, followed by their subsequent repair by various DNA repair pathways to generate sequence-diversified products. It is not known how either of the two steps is targeted specifically to Ig loci. Because of the tight link between transcription and SHM, we have investigated the role of endogenous Ig light chain (IgL) transcriptional control elements in GCV/SHM in the chicken B cell line DT40. Promoter substitution experiments led to identification of a strong RNA polymerase II promoter incapable of supporting efficient GCV/SHM. This surprising finding indicates that high levels of transcription are not sufficient for robust GCV/SHM in Ig loci. Deletion of the IgL enhancer in a context in which high-level transcription was not compromised showed that the enhancer is not necessary for GCV/SHM. Our results indicate that cis-acting elements are important for Ig gene diversification, and we propose that targeting specificity is achieved through the combined action of several Ig locus elements that include the promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.