ArticleMolecular and cellular biology2007
Sleeping beauty transposase has an affinity for heterochromatin conformation.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons.Biomedicines · 2025Review
- Tn5 DNA Transposase in Multi-Omics Research.Methods and protocols · 2023Review
- Review
- An efficient Screening System in Yeast to Select a HyperactiveInternational journal of molecular sciences · 2020Article
- Development of genome engineering technologies in cattle: from random to specific.Journal of animal science and biotechnology · 2018Review
- Chromatin states shape insertion profiles of the piggyBac, Tol2 and Sleeping Beauty transposons and murine leukemia virus.Scientific reports · 2017Article
- Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.Nature reviews. Genetics · 2017Review
- Review
- Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.Cellular and molecular life sciences : CMLS · 2015Review
- Exploiting the power of LINE-1 retrotransposon mutagenesis for identification of genes involved in embryonic stem cell differentiation.Stem cell reviews and reports · 2014Article
- DNA transposon-based gene vehicles - scenes from an evolutionary drive.Journal of biomedical science · 2013Review
- Regulation of DNA transposition by CpG methylation and chromatin structure in human cells.Mobile DNA · 2013Article
- The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites.Molecular and cellular biology · 2013Article
- Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis.Mobile DNA · 2011Article
- Retrotransposon-induced heterochromatin spreading in the mouse revealed by insertional polymorphisms.PLoS genetics · 2011Article
- Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transposition.Transgenic research · 2011Article
- A hyperactive piggyBac transposase for mammalian applications.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A transient transgenic RNAi strategy for rapid characterization of gene function during embryonic development.PloS one · 2010Article
- Harnessing transposons for cancer gene discovery.Nature reviews. Cancer · 2010Review
- An S/MAR-based L1 retrotransposition cassette mediates sustained levels of insertional mutagenesis without suffering from epigenetic silencing of DNA methylation.Epigenetics · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Sleeping Beauty (SB) transposase reconstructed from salmonid fish has high transposition activity in mammals and has been a useful tool for insertional mutagenesis and gene delivery. However, the transposition efficiency has varied significantly among studies. Our previous study demonstrated that the introduction of methylation into the SB transposon enhanced transposition, suggesting that transposition efficiency is influenced by the epigenetic status of the transposon region. Here, we examined the influence of the chromatin status on SB transposition in mouse embryonic stem cells. Heterochromatin conformation was introduced into the SB transposon by using a tetracycline-controlled transrepressor (tTR) protein, consisting of a tetracycline repressor (TetR) fused to the Kruppel-associated box (KRAB) domain of human KOX1 through tetracycline operator (tetO) sequences. The excision frequency of the SB transposon, which is the first step of the transposition event, was enhanced by approximately 100-fold. SB transposase was found to be colocalized with intense DAPI (4',6'-diamidino-2-phenylindole) staining and with the HP1 family by biochemical fractionation analyses. Furthermore, chromatin immunoprecipitation analysis revealed that SB transposase was recruited to tTR-induced heterochromatic regions. These data suggest that the high affinity of SB transposase for heterochromatin conformation leads to enhancement of SB transposition efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.