ArticleMolecular and cellular biology2007
Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- The choreography of chromatin in RNA polymerase III regulation.Biochemical Society transactions · 2024Review
- Locus-specific proteome decoding reveals Fpt1 as a chromatin-associated negative regulator of RNA polymerase III assembly.Molecular cell · 2023Article
- Conservation and Expansion of Transcriptional Factor Repertoire in theJournal of fungi (Basel, Switzerland) · 2023Article
- Conservation and Expansion of Transcriptional Factor Repertoire in thebioRxiv : the preprint server for biology · 2023Article
- Structural Features of the Nucleosomal DNA Modulate the Functional Binding of a Transcription Factor and Productive Transcription.Frontiers in genetics · 2022Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast.Nucleic acids research · 2017Article
- Promoter architecture and transcriptional regulation of Abf1-dependent ribosomal protein genes in Saccharomyces cerevisiae.Nucleic acids research · 2016Article
- Intergenic transcriptional interference is blocked by RNA polymerase III transcription factor TFIIIB in Saccharomyces cerevisiae.Genetics · 2014Article
- Investigating transcription reinitiation through in vitro approaches.Transcription · 2014Review
- A comprehensive genomic binding map of gene and chromatin regulatory proteins in Saccharomyces.Molecular cell · 2011Article
- Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation.Nucleic acids research · 2009Article
- TFIIIC binding sites function as both heterochromatin barriers and chromatin insulators in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- Genome-wide location analysis reveals a role of TFIIS in RNA polymerase III transcription.Genes & development · 2008Article
- Chromatin structure and expression of a gene transcribed by RNA polymerase III are independent of H2A.Z deposition.Molecular and cellular biology · 2008Article
- The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors.Cellular & molecular biology letters · 2008Article
- An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1.PloS one · 2007Article
- Nhp6: a small but powerful effector of chromatin structure in Saccharomyces cerevisiae.Biochimica et biophysica actaReview
- Regulation of pol III transcription by nutrient and stress signaling pathways.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A key event in tRNA gene (tDNA) transcription by RNA polymerase (Pol) III is the TFIIIC-dependent assembly of TFIIIB upstream of the transcription start site. Different tDNA upstream sequences bind TFIIIB with different affinities, thereby modulating tDNA transcription. We found that in the absence of Nhp6 proteins, the influence of the 5'-flanking region on tRNA gene transcription is dramatically enhanced in Saccharomyces cerevisiae. Expression of a tDNA bearing a suboptimal TFIIIB binding site, but not of a tDNA preceded by a strong TFIIIB binding region, was strongly dependent on Nhp6 in vivo. Upstream sequence-dependent stimulation of tRNA gene transcription by Nhp6 could be reproduced in vitro, and Nhp6 proteins were found associated with tRNA genes in yeast cells. We also show that both transcription and silencing barrier activity of a tDNA(Thr) at the HMR locus are compromised in the absence of Nhp6. Our data suggest that Nhp6 proteins are important components of Pol III chromatin templates that contribute both to the robustness of tRNA gene expression and to positional effects of Pol III transcription complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.