ArticleJournal of the American Chemical Society2006
Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays.
Article in Journal of the American Chemical Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 91 citations in OpenAlex.
- A Spellbinding Interplay Between Biological Barcoding and Nanotechnology.Frontiers in bioengineering and biotechnology · 2020Review
- Article
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
- Nanoplatforms for constructing new approaches to cancer treatment, imaging, and drug delivery: what should be the policy?NeuroImage · 2011Review
- Hybridization efficiency of molecular beacons bound to gold nanowires: effect of surface coverage and target length.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Organically modified silicas on metal nanowires.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Surface-enhanced Raman scattering biomedical applications of plasmonic colloidal particles.Journal of the Royal Society, Interface · 2010Review
- Nanostructures Enabled by On-Wire Lithography (OWL).Chemical physics letters · 2010Article
- Shape-coded silica nanotubes for multiplexed bioassay: rapid and reliable magnetic decoding protocols.Nanomedicine (London, England) · 2010Article
- Complementary electrical and spectroscopic detection assays with on-wire-lithography-based nanostructures.Small (Weinheim an der Bergstrasse, Germany) · 2009Article
- Nanowire sensors for multiplexed detection of biomolecules.Current opinion in chemical biology · 2008Review
- Molecular beacon-metal nanowire interface: effect of probe sequence and surface coverage on sensor performance.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- Nanodisk codes.Nano letters · 2007Article
- Using force spectroscopy analysis to improve the properties of the hairpin probe.Nucleic acids research · 2007Article
- Detection of human viral RNA via a combined fluorescence and SERS molecular beacon assay.Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have combined molecular beacon (MB) probes with barcoded metal nanowires to enable no-wash, sealed chamber, multiplexed detection of nucleic acids. Probe design and experimental parameters important in nanowire-based MB assays are discussed. Loop regions of 24 bases and 5 base pair stem regions in the beacon probes gave optimal performance. Our results suggest that thermodynamic predictions for secondary structure stability of solution-phase MB can guide probe design for nanowire-based assays. Dengue virus-specific probes with predicted solution-phase DeltaG of folding in 500 mM buffered NaCl of approximately -4 kcal/mol performed better than those with DeltaG > -2 or < -6 kcal/mol. Buffered 300-500 mM NaCl was selected after comparison of several buffers previously reported for similar types of assays, and 200-500 mM NaCl was found to be the optimal ionic strength for the hybridization temperatures (25 and 50 degrees C) and probe designs used here. Target binding to the surface as a function of solution concentration fit a Sips isotherm with Kd = 1.7 +/- 0.3 nM. The detection limit was approximately 100 pM, limited by incomplete quenching. Single base mismatches could be discriminated from fully complementary targets. Oligonucleotide target sequences specific for human immunodeficiency, hepatitis C, and severe acute respiratory viruses were assayed simultaneously in a no-wash, sealed chamber, multiplexed experiment in which each of three probe sequences was attached to a different pattern of encoded nanowires. Finally, we demonstrated that probe-coated nanowires retain their selectivity and sensitivity in a triplexed assay after storage for over 3 months.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.