ArticleBiochemistry2006
Mechanism of adenomatous polyposis coli (APC)-mediated blockage of long-patch base excision repair.
Article in Biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- In Vitro Reconstitutive Base Excision Repair (BER) Assay.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The splicing component ISY1 regulates APE1 in base excision repair.DNA repair · 2020Article
- APC loss affects DNA damage repair causing doxorubicin resistance in breast cancer cells.Neoplasia (New York, N.Y.) · 2019Article
- Multiple Roles of APC and its Therapeutic Implications in Colorectal Cancer.Journal of the National Cancer Institute · 2017Review
- Interacting partners of FEN1 and its role in the development of anticancer therapeutics.Oncotarget · 2017Review
- How does inflammation drive mutagenesis in colorectal cancer?Trends in cancer research · 2017Article
- A multigene mutation classification of 468 colorectal cancers reveals a prognostic role for APC.Nature communications · 2016Article
- Interaction between APC and Fen1 during breast carcinogenesis.DNA repair · 2016Review
- Genotoxicity of Cytolethal Distending Toxin (CDT) on Isogenic Human Colorectal Cell Lines: Potential Promoting Effects for Colorectal Carcinogenesis.Frontiers in cellular and infection microbiology · 2016Article
- Molecular mechanism of adenomatous polyposis coli-induced blockade of base excision repair pathway in colorectal carcinogenesis.Life sciences · 2015Review
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- 1,3-Bis(2-chloroethyl)-1-nitrosourea enhances the inhibitory effect of resveratrol on 5-fluorouracil sensitive/resistant colon cancer cells.World journal of gastroenterology · 2013Article
- Adenomatous polyposis coli-mediated accumulation of abasic DNA lesions lead to cigarette smoke condensate-induced neoplastic transformation of normal breast epithelial cells.Neoplasia (New York, N.Y.) · 2013Article
- Adenomatous polyposis coli interacts with flap endonuclease 1 to block its nuclear entry and function.Neoplasia (New York, N.Y.) · 2012Article
- Targeting DNA polymerase ß for therapeutic intervention.Current molecular pharmacology · 2012Review
- Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity.Biochemistry · 2011Article
- DNA polymerase β as a novel target for chemotherapeutic intervention of colorectal cancer.PloS one · 2011Article
- Molecular mechanisms of the whole DNA repair system: a comparison of bacterial and eukaryotic systems.Journal of nucleic acids · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recently, we found an interaction between adenomatous polyposis coli (APC) and DNA polymerase beta (pol-beta) and showed that APC blocks strand-displacement synthesis of long-patch base excision repair (LP-BER) (Narayan, S., Jaiswal, A. S., and Balusu, R. (2005) J. Biol. Chem. 280, 6942-6949); however, the mechanism is not clear. Using an in vivo LP-BER assay system, we now show that the LP-BER is higher in APC-/- cells than in APC+/+ cells. In addition to pol-beta, the pull-down experiments showed that the full-length APC also interacted with flap endonuclease 1 (Fen-1). To further characterize the interaction of APC with pol-beta and Fen-1, we performed a domain-mapping of APC and found that both pol-beta and Fen-1 interact with a 138-amino acids peptide from the APC at the DRI-domain. Our functional assays showed that APC blocks pol-beta-mediated 1-nucleotide (1-nt) as well as strand-displacement synthesis of reduced abasic, nicked-, or 1-nt gapped-DNA substrates. Further studies demonstrated that APC blocks 5'-flap endonuclease as well as the 5'-3' exonuclease activity of Fen-1 resulting in the blockage of LP-BER. From these results, we concluded that APC can have three different effects on the LP-BER pathway. First, APC can block pol-beta-mediated 1-nt incorporation and strand-displacement synthesis. Second, APC can block LP-BER by blocking the coordinated formation and removal of the strand-displaced flap. Third, APC can block LP-BER by blocking hit-and-run synthesis. These studies will have important implications for APC in DNA damage-induced carcinogenesis and chemoprevention.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.