ArticleNucleic acids research2007
Rapid DNA mapping by fluorescent single molecule detection.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 101 citations in OpenAlex.
- DOM: Dual Optical Mapping Integrating Sequence-Specific Barcodes and A/T Density Profiles.Small methods · 2026Article
- Sequencing and Optical Genome Mapping for the Adventurous Chemist.Chemical & biomedical imaging · 2024Review
- DNA Visualization Using Fluorescent Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
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- Multicolor Whole-Genome Mapping in Nanochannels for Genetic Analysis.Analytical chemistry · 2021Article
- Single-molecule telomere length characterization by optical mapping in nano-channel array: Perspective and review on telomere length measurement.Environmental toxicology and pharmacology · 2021Review
- Customized optical mapping by CRISPR-Cas9 mediated DNA labeling with multiple sgRNAs.Nucleic acids research · 2021Article
- High-Quality Genomes and High-Density Genetic Map Facilitate the Identification of Genes From a Weedy Rice.Frontiers in plant science · 2021Article
- Genomic methods for measuring DNA replication dynamics.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Comprehensive Analysis of Human Subtelomeres by Whole Genome Mapping.PLoS genetics · 2020Article
- A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules.Scientific reports · 2019Article
- Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.Frontiers in molecular biosciences · 2019Review
- Modelling BioNano optical data and simulation study of genome map assembly.Bioinformatics (Oxford, England) · 2018Article
- Reassessment of the evolution of wheat chromosomes 4A, 5A, and 7B.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Odijk excluded volume interactions during the unfolding of DNA confined in a nanochannel.Macromolecules · 2018Article
- Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly inMethodsX · 2018Article
- Enrichment of megabase-sized DNA molecules for single-molecule optical mapping and next-generation sequencing.Scientific reports · 2017Article
- High-throughput single-molecule telomere characterization.Genome research · 2017Article
- Ionic Current-Based Mapping of Short Sequence Motifs in Single DNA Molecules Using Solid-State Nanopores.Nano letters · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
DNA mapping is an important analytical tool in genomic sequencing, medical diagnostics and pathogen identification. Here we report an optical DNA mapping strategy based on direct imaging of individual DNA molecules and localization of multiple sequence motifs on the molecules. Individual genomic DNA molecules were labeled with fluorescent dyes at specific sequence motifs by the action of nicking endonuclease followed by the incorporation of dye terminators with DNA polymerase. The labeled DNA molecules were then stretched into linear form on a modified glass surface and imaged using total internal reflection fluorescence (TIRF) microscopy. By determining the positions of the fluorescent labels with respect to the DNA backbone, the distribution of the sequence motif recognized by the nicking endonuclease can be established with good accuracy, in a manner similar to reading a barcode. With this approach, we constructed a specific sequence motif map of lambda-DNA. We further demonstrated the capability of this approach to rapidly type a human adenovirus and several strains of human rhinovirus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.