ArticleThe Journal of cell biology1991
Fiber type- and position-dependent expression of a myosin light chain-CAT transgene detected with a novel histochemical stain for CAT.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 89 citations in OpenAlex.
- Mitochondrial and skeletal muscle health with advancing age.Molecular and cellular endocrinology · 2013Review
- Is functional hypertrophy and specific force coupled with the addition of myonuclei at the single muscle fiber level?FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Total skeletal muscle PGC-1 deficiency uncouples mitochondrial derangements from fiber type determination and insulin sensitivity.Cell metabolism · 2010Article
- The Pax3-Cre transgene exhibits a rostrocaudal gradient of expression in the skeletal muscle lineage.Genesis (New York, N.Y. : 2000) · 2009Article
- Human cytomegalovirus IE1 promoter/enhancer drives variable gene expression in all fiber types in transgenic mouse skeletal muscle.BMC genetics · 2000Article
- Myogenin induces a shift of enzyme activity from glycolytic to oxidative metabolism in muscles of transgenic mice.The Journal of cell biology · 1999Article
- Identification of a novel slow-muscle-fiber enhancer binding protein, MusTRD1.Molecular and cellular biology · 1998Article
- Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Muscle-specific transgenic complementation of GLUT4-deficient mice. Effects on glucose but not lipid metabolism.The Journal of clinical investigation · 1997Article
- Fibre type-specific and nerve-dependent regulation of myosin light chain 1 slow promoter in regenerating muscle.Journal of muscle research and cell motility · 1997Article
- A combination of MEF3 and NFI proteins activates transcription in a subset of fast-twitch muscles.Molecular and cellular biology · 1997Article
- Distinct regulatory elements control muscle-specific, fiber-type-selective, and axially graded expression of a myosin light-chain gene in transgenic mice.Molecular and cellular biology · 1996Article
- Common core sequences are found in skeletal muscle slow- and fast-fiber-type-specific regulatory elements.Molecular and cellular biology · 1996Article
- Independence and interdependence of the three human aldolase A promoters in transgenic mice.Gene expression · 1996Article
- Fast-muscle-specific DNA-protein interactions occurring in vivo at the human aldolase A M promoter are necessary for correct promoter activity in transgenic mice.Molecular and cellular biology · 1996Article
- Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle.Molecular and cellular biology · 1995Article
- Overexpression of myogenin in muscles of transgenic mice: interaction with Id-1, negative crossregulation of myogenic factors, and induction of extrasynaptic acetylcholine receptor expression.Molecular and cellular biology · 1995Article
- Activation of a muscle-specific enhancer by the Ski proto-oncogene.Nucleic acids research · 1995Article
- Delineation of a slow-twitch-myofiber-specific transcriptional element by using in vivo somatic gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Myosin light chain 3F regulatory sequences confer regionalized cardiac and skeletal muscle expression in transgenic mice.The Journal of cell biology · 1995Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We recently generated and characterized transgenic mice in which regulatory sequences from a myosin light chain gene (MLC1f/3f) are linked to the chloramphenicol acetyltransferase (CAT) gene. Transgene expression in these mice is specific to skeletal muscle and graded along the rostrocaudal axis: adult muscles derived from successively more caudal somites express successively higher levels of CAT. To investigate the cellular basis of these patterns of expression, we developed and used a histochemical stain that allows detection of CAT in individual cells. Our main results are as follows: (a) Within muscles, CAT is detected only in muscle fibers and not in associated connective tissue, blood vessels, or nerves. Thus, the tissue specificity of transgene expression observed by biochemical assay reflects a cell-type specificity demonstrable histochemically. (b) Within individual muscles, CAT levels vary with fiber type. Like the endogenous MLC1f/3f gene, the transgene is expressed at higher levels in fast-twitch (type II) than in slow-twitch (type I) muscle fibers. In addition, CAT levels vary among type II fiber subtypes, in the order IIB greater than IIX greater than IIA. (c) Among muscles that are similar in fiber type composition, the average level of CAT per fiber varies with rostrocaudal position. This position-dependent variation in CAT level is apparent even when fibers of a single type are compared. From these results, we conclude that fiber type and position affect CAT expression independently. We therefore infer the existence of separate fiber type-specific and positionally graded transcriptional regulators that act together to determine levels of transgene expression.
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