ArticleThe Journal of cell biology1991
Microtubule polymer assembly and transport during axonal elongation.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 164 citations in OpenAlex.
- Did axons evolve by activating cytokinesis during interphase? A hypothesis on the origin of neurons.Molecular biology of the cell · 2025Review
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.bioRxiv : the preprint server for biology · 2024Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.Frontiers in cell and developmental biology · 2024Article
- The role of mechanics in axonal stability and development.Seminars in cell & developmental biology · 2023Review
- Patterning Microtubule Network Organization Reshapes Cell-Like Compartments.ACS synthetic biology · 2021Article
- Axonal cytomechanics in neuronal development.Journal of biosciences · 2020Review
- The model of local axon homeostasis - explaining the role and regulation of microtubule bundles in axon maintenance and pathology.Neural development · 2019Review
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- Measurement of subcellular force generation in neurons.Biophysical journal · 2015Article
- Article
- Okadaic acid: more than a diarrheic toxin.Marine drugs · 2013Review
- Initial neurite outgrowth in Drosophila neurons is driven by kinesin-powered microtubule sliding.Current biology : CB · 2013Article
- Drosophila growth cones advance by forward translocation of the neuronal cytoskeletal meshwork in vivo.PloS one · 2013Article
- Identification of differentially expressed genes in SHSY5Y cells exposed to okadaic acid by suppression subtractive hybridization.BMC genomics · 2012Article
- The emerging role of forces in axonal elongation.Progress in neurobiology · 2011Review
- The role of stretching in slow axonal transport.Biophysical journal · 2011Article
- Physical model for the width distribution of axons.The European physical journal. E, Soft matter · 2009Article
- A physical model of axonal elongation: force, viscosity, and adhesions govern the mode of outgrowth.Biophysical journal · 2008Article
- Direct evidence for coherent low velocity axonal transport of mitochondria.The Journal of cell biology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As axons elongate, tubulin, which is synthesized in the cell body, must be transported and assembled into new structures in the axon. The mechanism of transport and the location of assembly are presently unknown. We report here on the use of tubulin tagged with a photoactivatable fluorescent group to investigate these issues. Photoactivatable tubulin, microinjected into frog embryos at the two-cell stage, is incorporated into microtubules in neurons obtained from explants of the neural tube. When activated by light, a fluorescent mark is made on the microtubules in the axon, and transport and turnover can be visualized directly. We find that microtubules are generated in or near the cell body and continually transported distally as a coherent phase of polymer during axon elongation. This vectorial polymer movement was observed at all levels on the axon, even in the absence of axonal elongation. Measurements of the rate of polymer translocation at various places in the axon suggest that new polymer is formed by intercalary assembly along the axon and assembly at the growth cone in addition to transport of polymer from the cell body. Finally, polymer movement near the growth cone appeared to respond in a characteristic manner to growth cone behavior, while polymer proximally in the axon moved more consistently. These results suggest that microtubule translocation is the principal means of tubulin transport and that translocation plays an important role in generating new axon structure at the growth cone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.