ArticleThe Journal of cell biology1991
Influence of receptor lateral mobility on adhesion strengthening between membranes containing LFA-3 and CD2.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 214 citations in OpenAlex.
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- Significance of Receptor Mobility in Multivalent Binding on Lipid Membranes.Angewandte Chemie (International ed. in English) · 2022Review
- Binding kinetics of liposome conjugated E-selectin and P-selectin glycoprotein ligand-1 measured with atomic force microscopy.Colloids and surfaces. B, Biointerfaces · 2021Article
- CD58 Immunobiology at a Glance.Frontiers in immunology · 2021Review
- Biomimetic particles as therapeutics.Trends in biotechnology · 2015Review
- Light and life in Baltimore--and beyond.Biophysical journal · 2015Article
- Ligand-mediated friction determines morphodynamics of spreading T cells.Biophysical journal · 2014Article
- Micropatterned, multicomponent supported lipid bilayers for cellular systems.Methods in cell biology · 2014Article
- Imaging of molecular surface dynamics in brain slices using single-particle tracking.Nature communications · 2014Article
- DNA-based patterning of tethered membrane patches.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Biophysical description of multiple events contributing blood leukocyte arrest on endothelium.Frontiers in immunology · 2013Article
- A spatial model for integrin clustering as a result of feedback between integrin activation and integrin binding.Biophysical journal · 2012Article
- An expression system for screening of proteins for glycan and protein interactions.Analytical biochemistry · 2011Article
- Lipopolymer gradient diffusion in supported bilayer membranes.Journal of the Royal Society, Interface · 2011Article
- Concentration dependence of lipopolymer self-diffusion in supported bilayer membranes.Journal of the Royal Society, Interface · 2011Article
- Novel cellular microarray assay for profiling T-cell peptide antigen specificities.Journal of proteome research · 2010Article
- Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides.Biomaterials · 2010Article
- Model lipid bilayer with facile diffusion of lipids and integral membrane proteins.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- DNA-mediated self-assembly of artificial vesicles.PloS one · 2010Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have used an in vitro model system of glass-supported planar membranes to study the effects of lateral mobility of membrane-bound receptors on cell adhesion. Egg phosphatidylcholine (PC) bilayers were reconstituted with two anchorage isoforms of the adhesion molecule lymphocyte function-associated antigen 3 (LFA-3). The diffusion coefficient of glycosyl phosphatidylinositol (GPI)-anchored LFA-3 approached that of phospholipids in the bilayers, whereas the transmembrane (TM)-anchored isoform of LFA-3 was immobile. Both static and laminar flow assays were used to quantify the strength of adherence to the lipid bilayers of the T lymphoma cell line Jurkat that expresses the counter-receptor CD2. Cell adhesion was dependent on LFA-3 density and was more efficient on membranes containing the GPI isoform than the TM isoform. Kinetic measurements demonstrated an influence of contact time on the strength of adhesion to the GPI isoform at lower site densities (25-50 sites/microns2), showing that the mobility of LFA-3 is important in adhesion strengthening. At higher site densities (1,500 sites/microns2) and longer contact times (20 min), Jurkat cell binding to the TM and GPI isoforms of LFA-3 showed equivalent adhesion strengths, although adhesion strength of the GPI isoform developed twofold more rapidly than the TM isoform. Reduction of CD2 mobility on Jurkat cells at 5 degrees C greatly decreased the rate of adhesion strengthening with the TM isoform of LFA-3, resulting in a 30-fold difference between the two LFA-3 isoforms. Our results demonstrate that the ability of a membrane receptor and its membrane-bound counter-receptor to diffuse laterally enhances cell adhesion both by allowing accumulation of ligands in the cell contact area and by increasing the rate of receptor-ligand bond formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.