ArticleEukaryotic cell2007
Analysis of Thalassiosira pseudonana silicon transporters indicates distinct regulatory levels and transport activity through the cell cycle.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 97 citations in OpenAlex.
- The morphogenetic activity of dAnk genes in the diatom Thalassiosira pseudonana is sensitive to Si availability.Communications biology · 2026Article
- Innovations in Silicon Transport Shaped the Rise of Biosilicification and Skeletal Evolution in Sponges.Molecular biology and evolution · 2026Article
- Phytoplankton phenology through gene expression during the North Atlantic spring bloom decline.ISME communications · 2026Article
- Three reference genomes for freshwater diatom ecology and evolution.Journal of phycology · 2025Article
- Molecular forecasting of domoic acid during a pervasive toxic diatom bloom.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bioaccumulation of Titanium in diatom Cyclotella atomus Hust.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Article
- Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation.Biotechnology for biofuels and bioproducts · 2023Article
- Characterization of the molecular mechanisms of silicon uptake in coccolithophores.Environmental microbiology · 2023Article
- Diminished carbon and nitrate assimilation drive changes in diatom elemental stoichiometry independent of silicification in an iron-limited assemblage.ISME communications · 2022Article
- Imaging and quantifying homeostatic levels of intracellular silicon in diatoms.Science advances · 2020Article
- A family of silicon transporter structural genes in a pennate diatom Synedra ulna subsp. danica (Kütz.) Skabitsch.PloS one · 2018Article
- Quantitative Proteomics Reveals Common and Specific Responses of a Marine DiatomFrontiers in microbiology · 2018Article
- Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.Microbial cell factories · 2017Article
- Dynamic responses to silicon in Thalasiossira pseudonana - Identification, characterisation and classification of signature genes and their corresponding protein motifs.Scientific reports · 2017Article
- Expression of Histophilus somni IbpA DR2 protective antigen in the diatom Thalassiosira pseudonana.Applied microbiology and biotechnology · 2017Article
- The Evolution of Silicon Transport in Eukaryotes.Molecular biology and evolution · 2016Article
- The evolution of silicon transporters in diatoms.Journal of phycology · 2016Article
- A model for the effects of germanium on silica biomineralization in choanoflagellates.Journal of the Royal Society, Interface · 2016Article
- Direct evidence of the molecular basis for biological silicon transport.Nature communications · 2016Article
- Transcript level coordination of carbon pathways during silicon starvation-induced lipid accumulation in the diatom Thalassiosira pseudonana.The New phytologist · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An analysis of the expression and activity of silicon transporters (SITs) was done on synchronously growing cultures of the diatom Thalassiosira pseudonana to provide insight into the role these proteins play in cellular silicon metabolism during the cell cycle. The first SIT-specific polyclonal peptide antibody was generated and used in the immunoblot analysis of whole-cell protein lysates to monitor SIT protein levels during synchronized progression through the cell cycle. Peaks in SIT protein levels correlated with active periods of silica incorporation into cell wall substructures. Quantitative real-time PCR on each of the three distinct SIT genes (TpSIT1, TpSIT2, and TpSIT3) showed that mRNA levels for the most highly expressed SIT genes peaked during the S phase of the cell cycle, a period prior to maximal silicon uptake and during which cell wall silicification does not occur. Variations in protein and mRNA levels did not correlate, suggesting that a significant regulatory step of SITs is at the translational or posttranslational level. Surge uptake rates also did not correlate with SIT protein levels, suggesting that SIT activity is internally controlled by the rate of silica incorporation. This is the first study to characterize SIT mRNA and protein expression and cellular uptake kinetics during the course of the cell cycle and cell wall synthesis, and it provides novel insight into SIT regulation.
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