ArticleThe Plant cell2006
Pollen tube tip growth depends on plasma membrane polarization mediated by tobacco PLC3 activity and endocytic membrane recycling.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 238 citations in OpenAlex.
- Loss of AtPLC1 Impairs Salt-Alkali Tolerance via Disruption of Stomatal Regulation and Redox Homeostasis inPlants (Basel, Switzerland) · 2026Article
- Quantitatively Analyzing the Structural Organization at the Tip of Growing Tobacco Pollen Tubes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Reciprocal regulation between AtFH5-labeled secretory vesicles and PI(4,5)PJournal of integrative plant biology · 2025Article
- Phosphoinositide Signaling and Actin Polymerization Are Critical for Tip Growth in the Marine Red AlgaPlants (Basel, Switzerland) · 2025Article
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- Genome-wide identification of the PI-PLC gene family inFrontiers in plant science · 2025Article
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- Exploring the Role ofInternational journal of molecular sciences · 2023Article
- Phosphoinositides in plant-pathogen interaction: trends and perspectives.Stress biology · 2023Review
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- Removal of the endoplasma membrane upon sperm cell activation after pollen tube discharge.Frontiers in plant science · 2023Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- Integrated Methylome and Transcriptome Analysis Provides Insights into the DNA Methylation Underlying the Mechanism of Cytoplasmic Male Sterility in Kenaf (International journal of molecular sciences · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Self-incompatibility requires GPI anchor remodeling by the poppy PGAP1 ortholog HLD1.Current biology : CB · 2022Article
- Article
- Emerging role of phospholipase C mediated lipid signaling in abiotic stress tolerance and development in plants.Plant cell reports · 2021Review
- ROP INTERACTIVE PARTNER b Interacts with RACB and Supports Fungal Penetration into Barley Epidermal Cells.Plant physiology · 2020Article
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana.Journal of experimental botany · 2020Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidyl inositol 4,5-bisphosphate (PI 4,5-P2) accumulates in a Rac/Rop-dependent manner in the pollen tube tip plasma membrane, where it may control actin organization and membrane traffic. PI 4,5-P2 is hydrolyzed by phospholipase C (PLC) activity to the signaling molecules inositol 1,4,5-trisphosphate and diacyl glycerol (DAG). To investigate PLC activity during tip growth, we cloned Nt PLC3, specifically expressed in tobacco (Nicotiana tabacum) pollen tubes. Recombinant Nt PLC3 displayed Ca2+-dependent PI 4,5-P2-hydrolyzing activity sensitive to U-73122 and to mutations in the active site. Nt PLC3 overexpression, but not that of inactive mutants, inhibited pollen tube growth. Yellow fluorescent protein (YFP) fused to Nt PLC3, or to its EF and C2 domains, accumulated laterally at the pollen tube tip plasma membrane in a pattern complementary to the distribution of PI 4,5-P2. The DAG marker Cys1:YFP displayed a similar intracellular localization as PI 4,5-P2. Blocking endocytic membrane recycling affected the intracellular distribution of DAG but not of PI 4,5-P2. U-73122 at low micromolar concentrations inhibited and partially depolarized pollen tube growth, caused PI 4,5-P2 spreading at the apex, and abolished DAG membrane accumulation. We show that Nt PLC3 is targeted by its EF and C2 domains to the plasma membrane laterally at the pollen tube tip and that it maintains, together with endocytic membrane recycling, an apical domain enriched in PI 4,5-P2 and DAG required for polar cell growth.
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