ArticleJournal of bacteriology2007
Functional analysis of PilT from the toxic cyanobacterium Microcystis aeruginosa PCC 7806.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Toxic/Bioactive Peptide Synthesis Genes Rearranged by Insertion Sequence Elements Among the Bloom-Forming CyanobacteriaFrontiers in microbiology · 2022Article
- The Role of the Cyanobacterial Type IV Pilus Machinery in Finding and Maintaining a Favourable Environment.Life (Basel, Switzerland) · 2020Review
- Genomics Approaches to Deciphering Natural Transformation in Cyanobacteria.Frontiers in microbiology · 2019Article
- Article
- A tight tunable range for Ni(II) sensing and buffering in cells.Nature chemical biology · 2017Article
- Genetic Identification of a PilT Motor inFrontiers in microbiology · 2016Article
- Integrating phylogeny, geographic niche partitioning and secondary metabolite synthesis in bloom-forming Planktothrix.The ISME journal · 2015Article
- Appendages of the cyanobacterial cell.Life (Basel, Switzerland) · 2015Review
- Nontoxic strains of cyanobacteria are the result of major gene deletion events induced by a transposable element.Molecular biology and evolution · 2008Article
- PilB and PilT are ATPases acting antagonistically in type IV pilus function in Myxococcus xanthus.Journal of bacteriology · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evolution of the microcystin toxin gene cluster in phylogenetically distant cyanobacteria has been attributed to recombination, inactivation, and deletion events, although gene transfer may also be involved. Since the microcystin-producing Microcystis aeruginosa PCC 7806 is naturally transformable, we have initiated the characterization of its type IV pilus system, involved in DNA uptake in many bacteria, to provide a physiological focus for the influence of gene transfer in microcystin evolution. The type IV pilus genes pilA, pilB, pilC, and pilT were shown to be expressed in M. aeruginosa PCC 7806. The purified PilT protein yielded a maximal ATPase activity of 37.5 +/- 1.8 nmol P(i) min(-1) mg protein(-1), with a requirement for Mg(2+). Heterologous expression indicated that it could complement the pilT mutant of Pseudomonas aeruginosa, but not that of the cyanobacterium Synechocystis sp. strain PCC 6803, which was unexpected. Differences in two critical residues between the M. aeruginosa PCC 7806 PilT (7806 PilT) and the Synechocystis sp. strain PCC 6803 PilT proteins affected their theoretical structural models, which may explain the nonfunctionality of 7806 PilT in its cyanobacterial counterpart. Screening of the pilT gene in toxic and nontoxic strains of Microcystis was also performed.
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