ArticleJournal of bacteriology2007
Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operon.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Disruption of the gene encoding restriction endonuclease SuaI and development of a host-vector system for the thermoacidophilic archaeon Sulfolobus acidocaldarius.Extremophiles : life under extreme conditions · 2016Article
- Identification of the minimal replicon and the origin of replication of the crenarchaeal plasmid pRN1.MicrobiologyOpen · 2014Article
- Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.Nucleic acids research · 2014Article
- Structure and function of AvtR, a novel transcriptional regulator from a hyperthermophilic archaeal lipothrixvirus.Journal of virology · 2013Article
- Functional characterization of the origin of replication of pRN1 from Sulfolobus islandicus REN1H1.PloS one · 2013Article
- Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.PloS one · 2013Article
- Simple and elegant design of a virion egress structure in Archaea.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcription termination in the plasmid/virus hybrid pSSVx from Sulfolobus islandicus.Extremophiles : life under extreme conditions · 2010Article
- Two novel families of plasmids from hyperthermophilic archaea encoding new families of replication proteins.Nucleic acids research · 2010Article
- DNA translocation activity of the multifunctional replication protein ORF904 from the archaeal plasmid pRN1.Nucleic acids research · 2009Article
- Structure, function, and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1.The Journal of biological chemistry · 2009Article
- Transcriptional analysis of the genetic element pSSVx: differential and temporal regulation of gene expression reveals correlation between transcription and replication.Journal of bacteriology · 2007Article
- Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi.Journal of bacteriology · 2007Article
- Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea.Nucleic acids research · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN. The plasmids in this family encode three conserved proteins that participate in plasmid replication and copy number regulation, as suggested by biochemical characterization of the recombinant proteins. In order to deepen our understanding of the molecular biology of these plasmids, we investigated the transcriptional activity of the model plasmid pRN1. We detected five major transcripts present at about 2 to 15 copies per cell. One long transcriptional unit comprises the genes for the plasmid-copy-number control protein Orf56/CopG and the replication protein Orf904. A second transcript with a long 3'-untranslated region codes for the DNA binding protein Orf80. For both transcripts, we identified countertranscripts which could play a regulatory role. The function of the fifth transcript is unclear. For the five transcripts, we determined the start site, the transcript end, the stability, and the abundance in different growth phases. Reporter gene experiments demonstrated that the copy number control protein Orf56 represses transcription of the orf56-orf904 cotranscript in vivo.
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