ArticlePlanta2007
Characterization of a beta-carotene hydroxylase of Adonis aestivalis and its expression in Arabidopsis thaliana.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Differential hydroxylation efficiency of the two non-heme carotene hydroxylases: DcBCH1, rather than DcBCH2, plays a major role in carrot taproot.Horticulture research · 2022Article
- Characterization and functional validation of β-carotene hydroxylaseHorticulture research · 2022Article
- Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana.BMC plant biology · 2021Article
- A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh.Plant physiology · 2015Article
- Article
- Functional implication of β-carotene hydroxylases in soybean nodulation.Plant physiology · 2013Article
- Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network.BMC plant biology · 2012Article
- Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus.Journal of experimental botany · 2012Article
- Lutein accumulation in the absence of zeaxanthin restores nonphotochemical quenching in the Arabidopsis thaliana npq1 mutant.The Plant cell · 2009Article
- Targeted modulation of sinapine biosynthesis pathway for seed quality improvement in Brassica napus.Transgenic research · 2009Article
- Metabolic engineering of novel ketocarotenoid production in carrot plants.Transgenic research · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoids are plant secondary metabolites that comprise two main groups: carotenes and xanthophylls. The latter group includes zeaxanthin which is synthesized by beta-carotene hydroxylase catalyzing the hydroxylation of the beta-rings of beta-carotene molecules. To develop tools to alter carotenoid biosynthesis in plants, we isolated a cDNA clone encoding a candidate beta-carotene hydroxylase, CrtH1, from the flower petals of Adonis aestivalis. CrtH1 protein has homology to beta-carotene hydroxylases from other organisms, and possesses the four histidine motifs conserved in this family of enzymes. Sequence analysis predicted the presence of a putative plastid transit peptide at the amino terminus and four transmembrane helical regions. Southern-blot analysis showed CrtH1 to be encoded by a multicopy gene family with at least three members in A. aestivalis. Analysis of CrtH1 transcript abundance by Northern blotting indicates it is highly expressed in flower petals, roots and stems, with relatively low expression in leaves and developing seeds. CrtH1 was able to catalyze the formation of zeaxanthin and its intermediate precursor beta-cryptoxanthin from beta-carotene in functional assays conducted in E. coli. Expression of CrtH1 in Arabidopsis thaliana wild type and a mutant deficient for endogenous beta-carotene hydroxylases enhanced the biosynthesis of violaxanthin in the seeds.
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Registered trials
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