ArticleThe Korean journal of parasitology2006
Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence.
Article in The Korean journal of parasitology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 48 citations in OpenAlex.
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- Characterization of novel extracellular proteases produced by Acanthamoeba castellanii after contact with human corneal epithelial cells and their relevance to pathogenesis.Parasites & vectors · 2024Article
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
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- Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3.Parasites & vectors · 2020Article
- Busting biofilms: free-living amoebae disrupt preformed methicillin-resistantMicrobiology (Reading, England) · 2020Article
- Extracellular protease profile of Acanthamoeba after prolonged axenic culture and after interaction with MDCK cells.Parasitology research · 2020Article
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- Characterization of extracellular proteases of Acanthamoeba genotype T4 isolated from different sources in Iran.Parasitology research · 2017Article
- Diversity, Structures, and Collagen-Degrading Mechanisms of Bacterial Collagenolytic Proteases.Applied and environmental microbiology · 2015Review
- Characterization of a novel subtilisin-like protease myroicolsin from deep sea bacterium Myroides profundi D25 and molecular insight into its collagenolytic mechanism.The Journal of biological chemistry · 2014Article
- Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2.PloS one · 2014Article
- Morpho-Physiological and Biochemical Criteria of Acanthamoeba spp. Isolated from the Egyptian Aquatic Environment.Iranian journal of parasitology · 2013Article
- Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors.Journal of tropical medicine · 2013Article
- Host-parasite interaction: parasite-derived and -induced proteases that degrade human extracellular matrix.Journal of parasitology research · 2012Article
- Serine protease activities in Leishmania (Leishmania) chagasi promastigotes.Parasitology research · 2010Article
- Acanthamoeba culbertsoni elicits soluble factors that exert anti-microglial cell activity.Infection and immunity · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenic mechanism of granulomatous amebic encephalitis (GAE) and amebic keratitis (AK) by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the pathogenesis of GAE and AK. In the present study, we have compared specific activity and cytopathic effects (CPE) of purified 33 kDa serine proteinases from Acanthamoeba strains with different degree of virulence (A. healyi OC-3A, A. lugdunensis KA/E2, and A. castellanii Neff). Trophozoites of the 3 strains revealed different degrees of CPE on human corneal epithelial (HCE) cells. The effect was remarkably reduced by adding phenylmethylsulfonylfluoride (PMSF), a serine proteinase inhibitor. This result indicated that PMSF-susceptible proteinase is the main component causing cytopathy to HCE cells by Acanthamoeba. The purified 33 kDa serine proteinase showed strong activity toward HCE cells and extracellular matrix proteins. The purified proteinase from OC-3A, the most virulent strain, demonstrated the highest enzyme activity compared to KA/E2, an ocular isolate, and Neff, a soil isolate. Polyclonal antibodies against the purified 33 kDa serine proteinase inhibit almost completely the proteolytic activity of culture supernatant of Acanthamoeba. In line with these results, the 33 kDa serine proteinase is suggested to play an important role in pathogenesis and to be the main component of virulence factor of Acanthamoeba.
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