ArticleProceedings of the National Academy of Sciences of the United States of America2006
Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
76 citing papers in PubMed, 194 citations in OpenAlex.
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- Next generation sequencing and beyond: a review of genomic sequencing methods.Functional & integrative genomics · 2025Article
- Template-independent enzymatic synthesis of RNA oligonucleotides.Nature biotechnology · 2025Article
- Investigation of simple BODIPY dyes as G-quadruplex recognizing ligands.RSC advances · 2025Article
- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
- Controlled enzymatic synthesis of oligonucleotides.Communications chemistry · 2024Review
- Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition.Analytical chemistry · 2023Article
- Identification of the Efficacy of Ex Situ Conservation ofPlants (Basel, Switzerland) · 2023Article
- Biocatalytic Synthesis of Antiviral Nucleosides, Cyclic Dinucleotides, and Oligonucleotide Therapies.JACS Au · 2023Review
- Towards the controlled enzymatic synthesis of LNA containing oligonucleotides.Frontiers in chemistry · 2023Article
- Fluorophore-mediated Photooxidation of the Guanine Heterocycle.Journal of physical organic chemistry · 2022Article
- Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencing.Life science alliance · 2022Article
- Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA.RSC advances · 2022Article
- Review
- Genome sequencing guide: An introductory toolbox to whole-genome analysis methods.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2021Review
- Uncertainties in synthetic DNA-based data storage.Nucleic acids research · 2021Review
- DNA bridges: A novel platform for single-molecule sequencing and other DNA-protein interaction applications.PloS one · 2021Article
- Exploiting Single-Cell Tools in Gene and Cell Therapy.Frontiers in immunology · 2021Review
- Nucleotide analogues as inhibitors of SARS-CoV Polymerase.Pharmacology research & perspectives · 2020Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
DNA sequencing by synthesis (SBS) on a solid surface during polymerase reaction offers a paradigm to decipher DNA sequences. We report here the construction of such a DNA sequencing system using molecular engineering approaches. In this approach, four nucleotides (A, C, G, T) are modified as reversible terminators by attaching a cleavable fluorophore to the base and capping the 3'-OH group with a small chemically reversible moiety so that they are still recognized by DNA polymerase as substrates. We found that an allyl moiety can be used successfully as a linker to tether a fluorophore to 3'-O-allyl-modified nucleotides, forming chemically cleavable fluorescent nucleotide reversible terminators, 3'-O-allyl-dNTPs-allyl-fluorophore, for application in SBS. The fluorophore and the 3'-O-allyl group on a DNA extension product, which is generated by incorporating 3'-O-allyl-dNTPs-allyl-fluorophore in a polymerase reaction, are removed simultaneously in 30 s by Pd-catalyzed deallylation in aqueous buffer solution. This one-step dual-deallylation reaction thus allows the reinitiation of the polymerase reaction and increases the SBS efficiency. DNA templates consisting of homopolymer regions were accurately sequenced by using this class of fluorescent nucleotide analogues on a DNA chip and a four-color fluorescent scanner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.