ArticleNucleic acids research2007
Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 87 citations in OpenAlex.
- Peptide-Based Plasmon-Enhanced Spectroscopic Immunoassay to Detect Immunity Against Cytomegalovirus.Biosensors · 2025Article
- Plasmonic nanoparticle sensors: current progress, challenges, and future prospects.Nanoscale horizons · 2024Review
- Nanomaterial-Based Fluorescence Resonance Energy Transfer (FRET) and Metal-Enhanced Fluorescence (MEF) to Detect Nucleic Acid in Cancer Diagnosis.Biomedicines · 2021Review
- Recent Advances in Biosensing With Photonic Crystal Surfaces: A Review.IEEE sensors journal · 2016Article
- Enhanced light collection in fluorescence microscopy using self-assembled micro-reflectors.Scientific reports · 2015Article
- Shrink-induced silica multiscale structures for enhanced fluorescence from DNA microarrays.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Photonic crystal enhancement of a homogeneous fluorescent assay using submicron fluid channels fabricated by E-jet patterning.Journal of biophotonics · 2014Article
- Hybrid silver nanoparticle/conjugated polyelectrolyte nanocomposites exhibiting controllable metal-enhanced fluorescence.Scientific reports · 2014Article
- Spatially selective photonic crystal enhanced fluorescence and application to background reduction for biomolecule detection assays.Optics express · 2011Article
- Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range.Nature communications · 2011Article
- Improved sensitivity of DNA microarrays using photonic crystal enhanced fluorescence.Analytical chemistry · 2010Article
- Metal-Enhanced Fluorescence (MEF): Physical Characterization of Siver-Island Films and Exploring Sample Geometries.Chemical physics letters · 2009Article
- A detection instrument for enhanced-fluorescence and label-free imaging on photonic crystal surfaces.Optics express · 2009Article
- Nanomaterial-assisted signal enhancement of hybridization for DNA biosensors: a review.Sensors (Basel, Switzerland) · 2009Article
- Plasmon-controlled fluorescence towards high-sensitivity optical sensing.Advances in biochemical engineering/biotechnology · 2009Review
- Microarray-based estimation of SNP allele-frequency in pooled DNA using the Langmuir kinetic model.BMC genomics · 2008Article
- Enhanced fluorescence images for labeled cells on silver island films.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- Fluorescence signal amplification on the gel biochips with a mirror surface and optimization of immunoassay procedure.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The effects of metal-enhanced fluorescence (MEF) have been measured for two dyes commonly used in DNA microarrays, Cy3 and Cy5. Silver island films (SIFs) grown on glass microscope slides were used as substrates for MEF DNA arrays. We examined MEF by spotting biotinylated, singly-labeled 23 bp DNAs onto avidin-coated SIF substrates. The fluorescence enhancement was found to be dependent on the DNA spotting concentration: below approximately 12.5 muM, MEF increased linearly, and at higher concentrations MEF remained at a constant maximum of 28-fold for Cy5 and 4-fold for Cy3, compared to avidin-coated glass substrates. Hybridization of singly-labeled oligonucleotides to arrayed single-stranded probes showed lower maximal MEF factors of 10-fold for Cy5 and 2.5-fold for Cy3, because of the smaller amount of immobilized fluorophores as a result of reduced surface hybridization efficiencies. We discuss how MEF can be used to increase the sensitivity of DNA arrays, especially for far red emitting fluorophores like Cy5, without significantly altering current microarray protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.