ArticleNucleic acids research2007
Selenium derivatization of nucleic acids for crystallography.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
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- Heavy Chalcogen Properties of Sulfur and Selenium Enhance Nucleic Acid-Based Therapeutics.Biomolecules · 2025Review
- Mechanism of the extremely high duplex-forming ability of oligonucleotides modified with N-tert-butylguanidine- or N-tert-butyl-N'- methylguanidine-bridged nucleic acids.Nucleic acids research · 2023Article
- General Strategies for RNA X-ray Crystallography.Molecules (Basel, Switzerland) · 2023Review
- Tellurium-Modified Nucleosides, Nucleotides, and Nucleic Acids with Potential Applications.Molecules (Basel, Switzerland) · 2022Review
- Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging.Nature communications · 2021Article
- Construction and structure studies of DNA-bipyridine complexes as versatile scaffolds for site-specific incorporation of metal ions into DNA.Journal of biomolecular structure & dynamics · 2019Article
- Temperature-Induced Replacement of Phosphate Proton with Metal Ion Captured in Neutron Structures of A-DNA.Structure (London, England : 1993) · 2018Article
- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data.Methods in molecular biology (Clifton, N.J.) · 2016Article
- The importance of fitting in: conformational preference of selenium 2' modifications in nucleosides and helical structures.Journal of biomolecular structure & dynamics · 2015Article
- Structure-based DNA-targeting strategies with small molecule ligands for drug discovery.Medicinal research reviews · 2013Review
- Synthesis of 6-Se-guanosine RNAs for structural study.Organic letters · 2013Article
- Synthesis of novel di-Se-containing thymidine and Se-DNAs for structure and function studies.Science China. Chemistry · 2013Article
- Hydrogen bond formation between the naturally modified nucleobase and phosphate backbone.Nucleic acids research · 2012Article
- Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.Current protocols in nucleic acid chemistry · 2011Article
- Facile synthesis of nucleoside 5'-(α-P-seleno)-triphosphates and phosphoroselenoate RNA transcription.RNA (New York, N.Y.) · 2011Article
- Synthesis, structure and imaging of oligodeoxyribonucleotides with tellurium-nucleobase derivatization.Nucleic acids research · 2011Article
- A fast selenium derivatization strategy for crystallization and phasing of RNA structures.RNA (New York, N.Y.) · 2009Article
- Derivatization of DNAs with selenium at 6-position of guanine for function and crystal structure studies.Nucleic acids research · 2008Article
- Selenium derivatization of nucleic acids for phase and structure determination in nucleic acid X-ray crystallography.International journal of molecular sciences · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high-resolution structure of the DNA (5'-GTGTACA-C-3') with the selenium derivatization at the 2'-position of T2 was determined via MAD and SAD phasing. The selenium-derivatized structure (1.28 A resolution) with the 2'-Se modification in the minor groove is isomorphorous to the native structure (2.0 A). To directly compare with the conventional bromine derivatization, we incorporated bromine into the 5-postion of T4, determined the bromine-derivatized DNA structure at 1.5 A resolution, and found that the local backbone torsion angles and solvent hydration patterns were altered in the structure with the Br incorporation in the major groove. Furthermore, while the native and Br-derivatized DNAs needed over a week to form reasonable-size crystals, we observed that the Se-derivatized DNAs grew crystals overnight with high-diffraction quality, suggesting that the Se derivatization facilitated the crystal formation. In addition, the Se-derivatized DNA sequences crystallized under a broader range of buffer conditions, and generally had a faster crystal growth rate. Our experimental results indicate that the selenium derivatization of DNAs may facilitate the determination of nucleic acid X-ray crystal structures in phasing and high-quality crystal growth. In addition, our results suggest that the Se derivatization can be an alternative to the conventional Br derivatization.
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