ArticleJournal of clinical microbiology2007
A cautionary tale: Lack of consistency in allele sizes between two laboratories for a published multilocus microsatellite typing system.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed.
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping of Aedes aegypti.Parasites & vectors · 2026Article
- Data collected in a citizen scientist study uncover a new species record of Phoxinus minnow for Austria.Environmental monitoring and assessment · 2026Article
- Efficient amplicon panels for evaluating hybridization and genetic structure in the Schistosoma haematobium species group.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2026Article
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping ofbioRxiv : the preprint server for biology · 2025Article
- Development of a Noninvasive Genotyping-In-Thousands (GTseq) Panel for Long-Term Conservation of Western Great Lakes Gray Wolves (Ecology and evolution · 2025Article
- A new GTSeq resource to facilitate multijurisdictional research and management of walleyeEcology and evolution · 2022Article
- A refined panel of 42 microsatellite loci to universally genotype catarrhine primates.Ecology and evolution · 2021Article
- Comparison of MultiLocus Sequence Typing (MLST) and Microsatellite Length Polymorphism (MLP) forComputational and structural biotechnology journal · 2020Article
- Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.Frontiers in microbiology · 2020Article
- Multilocus Variable-Number Tandem-Repeat Analysis of Yersinia ruckeri Confirms the Existence of Host Specificity, Geographic Endemism, and Anthropogenic Dissemination of Virulent Clones.Applied and environmental microbiology · 2018Article
- CHIIMP: An automated high-throughput microsatellite genotyping platform reveals greater allelic diversity in wild chimpanzees.Ecology and evolution · 2018Article
- High-Throughput Sequencing Strategy for Microsatellite Genotyping Using Neotropical Fish as a Model.Frontiers in genetics · 2018Article
- Next Gen Pop Gen: implementing a high-throughput approach to population genetics in boarfish (Royal Society open science · 2016Article
- Species identification, host range and diversity of Cecidophyopsis mites (Acari: Trombidiformes) infesting Ribes in Latvia.Experimental & applied acarology · 2016Article
- A novel polymorphic repeat in the upstream regulatory region of the estrogen-induced gene EIG121 is not associated with the risk of developing breast or endometrial cancer.BMC research notes · 2016Article
- A polymorphic repeat in the IGF1 promoter influences the risk of endometrial cancer.Endocrine connections · 2016Article
- A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding.Royal Society open science · 2016Article
- Development of a framework for genotyping bovine-derived Cryptosporidium parvum, using a multilocus fragment typing tool.Parasites & vectors · 2015Article
- Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Molecular Subtyping of Campylobacter jejuni by Using Capillary Electrophoresis.Applied and environmental microbiology · 2015Article
- A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti.G3 (Bethesda, Md.) · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
For species with low genetic diversity, typing using the differences in PCR fragment length resulting from variations in numbers of short tandem repeats has been shown to provide a high level of discrimination. This technique has been called multilocus microsatellite typing (MLMT) or multiple-locus variable-number tandem repeat analysis, and studies usually employ genetic or sequence analyzers to size PCR fragments to a high degree of precision. We set out to validate one such system that has been developed for Aspergillus fumigatus (H. A. de Valk, J. F. G. M. Meis, I. M. Curfs, K. Muehlethaler, J. W. Mouton, and C. H. W. Klaassen, J. Clin. Microbiol. 43:4112-4120, 2005). The sizes of the alleles were compared both by sequencing and from two genotyping laboratories, where they used capillary electrophoresis (CE) for sizing. Size differences of up to 6 bases were found between the actual sizes reported by sequencing and the sizes reported by CE. In addition, because the two genotyping laboratories used different machines and running conditions, differences of up to 3 bases were identified between them. As the microsatellite markers used differ by repeat units of 3 or 4 bases, it was not possible to assign PCR fragments to the correct alleles without confirming the sizes of a range of alleles by direct sequencing. Lines of best fit were plotted for each CE machine against actual sizes and will therefore enable unsequenced PCR fragments to be assigned to the correct alleles. This study highlights the care required to ensure that an MLMT system undergoes a suitable correction procedure before data can be merged between different laboratories involved in the typing of individual species.
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Registered trials
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