ArticleJournal of virology2007
Influenza virion-derived viral ribonucleoproteins synthesize both mRNA and cRNA in vitro.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 76 citations in OpenAlex.
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- The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.Nature communications · 2024Article
- Twenty natural amino acid substitution screening at the last residue 121 of influenza A virus NS2 protein reveals the critical role of NS2 in promoting virus genome replication by coordinating with viral polymerase.Journal of virology · 2024Article
- Defining the minimal components of the influenza A virus replication machinery via an in vitro reconstitution system.PLoS biology · 2023Article
- Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis.Communications biology · 2021Article
- Segment-specific kinetics of mRNA, cRNA and vRNA accumulation during influenza infection.Journal of virology · 2021Article
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- Structural insights into influenza A virus ribonucleoproteins reveal a processive helical track as transcription mechanism.Nature microbiology · 2020Article
- The Surface-Exposed PAJournal of virology · 2018Article
- A Mechanism for Priming and Realignment during Influenza A Virus Replication.Journal of virology · 2018Article
- Alternative reverse genetics system for influenza viruses based on a synthesized swine 45S rRNA promoter.Virus genes · 2017Article
- Unexpected complexity in the interference activity of a cloned influenza defective interfering RNA.Virology journal · 2017Article
- An in vitro fluorescence based study of initiation of RNA synthesis by influenza B polymerase.Nucleic acids research · 2017Article
- Influenza virus RNA polymerase: insights into the mechanisms of viral RNA synthesis.Nature reviews. Microbiology · 2016Review
- Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9).International journal of molecular sciences · 2015Article
- pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA.eLife · 2015Article
- A novel functional site in the PB2 subunit of influenza A virus essential for acetyl-CoA interaction, RNA polymerase activity, and viral replication.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The mechanisms regulating the synthesis of mRNA, cRNA, and viral genomic RNA (vRNA) by the influenza A virus RNA-dependent RNA polymerase are not fully understood. Early results suggested that the RNA polymerase "switched" from a transcriptase to a replicase during the viral life cycle in response to the expression of viral proteins. However, recently an alternative model suggesting that replication of influenza virus is regulated by stabilization of the replicative intermediates was proposed. According to this model, the virion-associated polymerase is capable of synthesizing both mRNA and cRNA. We now demonstrate that virion-derived viral ribonucleoproteins (vvRNPs) synthesize both mRNA and cRNA in vitro in the absence of non-virion-associated RNA polymerase or nucleoproteins. The authenticity of the in vitro-transcribed mRNA and cRNA was confirmed by terminal sequence analysis. The addition of non-virion-associated polymerase or NP had no effect on vvRNP activity. De novo synthesis of cRNA was found to be more sensitive than the capped primer-dependent synthesis of mRNA to the concentration of ATP, CTP, and GTP. We conclude that vvRNPs intrinsically possess both transcriptase and replicase activities and that there is no switch in the synthesis of mRNA to cRNA during the influenza virus life cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.