ArticleNucleic acids research2007
A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A role for worm cutl-24 in background- and parent-of-origin-dependent ER stress resistance.BMC genomics · 2022Article
- TheGenetics · 2019Review
- Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes.PLoS genetics · 2016Article
- Quantitative and automated high-throughput genome-wide RNAi screens in C. elegans.Journal of visualized experiments : JoVE · 2012Article
- A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.PloS one · 2012Article
- Identification of factors required for meristem function in Arabidopsis using a novel next generation sequencing fast forward genetics approach.BMC genomics · 2011Article
- Reverse genetics in eukaryotes.Biology of the cell · 2010Review
- Article
- The NemaGENETAG initiative: large scale transposon insertion gene-tagging in Caenorhabditis elegans.Genetica · 2009Review
- Single-copy insertion of transgenes in Caenorhabditis elegans.Nature genetics · 2008Article
- Article
- From genes to function: the C. elegans genetic toolbox.Wiley interdisciplinary reviews. Developmental biologyReview
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation of a large collection of defined transposon insertion mutants is of general interest to the Caenorhabditis elegans research community and has been supported by the European Union. We describe here a semi-automated high-throughput method for mutant production and screening, using the heterologous transposon Mos1. The procedure allows routine culture of several thousand independent nematode strains in parallel for multiple generations before stereotyped molecular analyses. Using this method, we have already generated >17 500 individual strains carrying Mos1 insertions. It could be easily adapted to forward and reverse genetic screens and may influence researchers faced with making a choice of model organism.
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