ArticleIn vitro cellular & developmental biology. Animal
Differentiation of prostate epithelial cell cultures by matrigel/ stromal cell glandular reconstruction.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Integrated analysis of single-cell and bulk transcriptomes uncovers clinically relevant molecular subtypes in human prostate cancer.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2025Article
- Failure to progress: breast and prostate cancer cell lines in developing targeted therapies.Cancer metastasis reviews · 2024Review
- Characterisation of cell lines derived from prostate cancer patients with localised disease.Prostate cancer and prostatic diseases · 2023Article
- Single luminal epithelial progenitors can generate prostate organoids in culture.Nature cell biology · 2014Article
- DNA hypermethylation in prostate cancer is a consequence of aberrant epithelial differentiation and hyperproliferation.Cell death and differentiation · 2014Article
- Conserved two-step regulatory mechanism of human epithelial differentiation.Stem cell reports · 2014Article
- Nuclear targeting of dystroglycan promotes the expression of androgen regulated transcription factors in prostate cancer.Scientific reports · 2013Article
- Article
- A transductionally retargeted adenoviral vector for virotherapy of Her2/neu-expressing prostate cancer.Human gene therapy · 2012Article
- Estrogen-initiated transformation of prostate epithelium derived from normal human prostate stem-progenitor cells.Endocrinology · 2011Article
- Modeling the prostate stem cell niche: an evaluation of stem cell survival and expansion in vitro.Stem cells and development · 2010Article
- Polarized fluid movement and not cell death, creates luminal spaces in adult prostate epithelium.Cell death and differentiation · 2009Article
- Identification of secreted glycoproteins of human prostate and bladder stromal cells by comparative quantitative proteomics.The Prostate · 2009Article
- Inhibition of monoamine oxidase A promotes secretory differentiation in basal prostatic epithelial cells.Differentiation; research in biological diversity · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Three-dimensional epithelial culture models are widely used to emulate a more physiologically relevant microenvironment for the study of genes and signaling pathways. Prostate epithelial cells can grow into solid cell masses or acinus-like spheroids in Matrigel. To test if the ability to form acinus-like spheroids in Matrigel is dependent on how undifferentiated a cell is or whether it is tumor or nontumor, we established six novel epithelial cell lines. Primary prostate epithelial cells were immortalized using HPV16 E6 gene transduction and were named Shmac 2, 3, and 6 (nontumor); Shmac 4, Shmac 5, and P4E6 (tumor). All cell lines were phenotyped in monolayer culture, and their ability to form acinus-like spheroids in Matrigel investigated. The cell lines exhibited a wide range of population doubling times and all showed an intermediate phenotype in monolayer culture ((luminal)CK(+)/(basal)CK(+)/CD44(+)/PSA(+)/AR(-)). Only Shmac 5 cells formed acinus-like spheroids when cultured in Matrigel. Co-culture of the spheroids with fibroblasts advanced differentiation by inducing androgen receptor expression and epithelial polarization. Our findings indicate that tumor cells can form acinus-like spheroids in Matrigel.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.