ArticleThe EMBO journal2007
Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 117 citations in OpenAlex.
- WormTagDB: a systematic survey of endogenously tagged proteins in Caenorhabditis elegans and roadmap toward the tagged proteome.G3 (Bethesda, Md.) · 2026Article
- Tissue-specific DNA repair strategies underlie apparent high transposon activity in the Caenorhabditis elegans soma.G3 (Bethesda, Md.) · 2026Article
- WormTagDB: A Systematic Survey of Endogenously Tagged Proteins inbioRxiv : the preprint server for biology · 2025Article
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- Silencing of a NRDE-3 transgene inmicroPublication biology · 2024Article
- Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators.Plant reproduction · 2023Review
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Detection of homolog-independent meiotic DNA repair events inSTAR protocols · 2021Article
- Meiotic DNA break repair can utilize homolog-independent chromatid templates in C. elegans.Current biology : CB · 2021Article
- Crossover Position Drives Chromosome Remodeling for Accurate Meiotic Chromosome Segregation.Current biology : CB · 2020Article
- Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans.PloS one · 2020Article
- Visualization and Quantification of Transposon Activity inG3 (Bethesda, Md.) · 2019Article
- The Claudin-like Protein HPO-30 Is Required to Maintain LAChRs at theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Reliable CRISPR/Cas9 Genome Engineering inG3 (Bethesda, Md.) · 2017Article
- Article
- Preventing Illegitimate Extrasynaptic Acetylcholine Receptor Clustering Requires the RSU-1 Protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Polq-Mediated End Joining Is Essential for Surviving DNA Double-Strand Breaks during Early Zebrafish Development.Cell reports · 2016Article
- Review
- Genome Editing in C. elegans and Other Nematode Species.International journal of molecular sciences · 2016Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template. Consequently, mutations engineered in the transgene could be copied to a specific locus at high frequency. This pathway was further characterized to develop an efficient tool--called MosTIC--to manipulate the C. elegans genome. Analysis of DSB repair during MosTIC experiments demonstrated that DSBs could also be sealed by end-joining in the germ line, independently from the evolutionarily conserved Ku80 and ligase IV factors. In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.