ArticleProceedings of the National Academy of Sciences of the United States of America2006
Evolutionary constraint on Otx2 neuroectoderm enhancers-deep conservation from skate to mouse and unique divergence in teleost.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
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- CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development.Frontiers in neuroanatomy · 2017Article
- Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation.International journal of biological sciences · 2015Article
- CentrinFish permit the visualization of centrosome dynamics in a cellular context in vivo.Zebrafish · 2014Article
- Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors.Molecular and cellular biology · 2012Article
- Ancient Pbx-Hox signatures define hundreds of vertebrate developmental enhancers.BMC genomics · 2011Article
- Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic position.EvoDevo · 2010Article
- Early evolution of conserved regulatory sequences associated with development in vertebrates.PLoS genetics · 2009Article
- Acetylated YY1 regulates Otx2 expression in anterior neuroectoderm at two cis-sites 90 kb apart.The EMBO journal · 2007Article
- Rapid and efficient generation of cartilage pellets from mouse induced pluripotent stem cells by transcriptional activation of BMP-4 with shaking culture.Journal of tissue engineeringArticle
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Authors and funding
9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Otx2 is a paired type homeobox gene that plays essential roles in each step and site of head development in vertebrates. In the mouse, Otx2 expression in the anterior neuroectoderm is regulated primarily by two distinct enhancers: anterior neuroectoderm (AN) and forebrain/midbrain (FM) enhancers at 92 kb and 75 kb 5'of the Otx2 locus, respectively. The AN enhancer has activity in the entire anterior neuroectoderm at headfold and early somite stages, whereas the FM enhancer is subsequently active in the future caudal forebrain and midbrain ectoderm. In tetrapods, both AN and FM enhancers are conserved, whereas the AN region is missing in teleosts, despite overt Otx2 expression in the anterior neuroectoderm. Here, we show that zebrafish and fugu FM regions drive expression not only in the forebrain and midbrain but also in the anterior neuroectoderm at headfold stage. The analysis of coelacanth and skate genomic Otx2 orthologues suggests that the utilization of the two enhancers, AN and FM, is an ancestral condition. In contrast, the AN enhancer has been specifically lost in the teleost lineage with a compensatory establishment of AN activity within the FM enhancer. Furthermore, the AN activity in the fish FM enhancer was established by recruiting upstream factors different from those that direct the tetrapod AN enhancer, yet zebrafish FM enhancer is active in both mouse and zebrafish anterior neuroectoderm at the headfold stage.
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