ArticleProceedings of the National Academy of Sciences of the United States of America2006
Flux control and excess capacity in the enzymes of glycolysis and their relationship to flight metabolism in Drosophila melanogaster.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- Arginine Kinase 1 supports energy homeostasis in Drosophila flight muscle development.PLoS genetics · 2026Article
- Molecular evolution in light of regulatory-coding epistasis.EMBO reports · 2026Review
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Lipid Metabolism in Relation to Carbohydrate Metabolism.Advances in experimental medicine and biology · 2026Review
- Insect Flight Energetics and the Evolution of Size, Form, and Function.Integrative and comparative biology · 2024Review
- Tissue-specific metabolic enzyme levels covary with whole-animal metabolic rates and life-history loci via epistatic effects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- REPTOR and CREBRF encode key regulators of muscle energy metabolism.Nature communications · 2023Article
- ChIP-seq profiling of H3K4me3 and H3K27me3 in an invasive insect,Frontiers in genetics · 2023Article
- Genome-wide transcriptomic changes reveal the genetic pathways involved in insect migration.Molecular ecology · 2022Article
- Homeostatic medicine: a strategy for exploring health and disease.Current medicine (Cham, Switzerland) · 2022Review
- A primer on harnessing non-enzymatic post-translational modifications for drug design.RSC medicinal chemistry · 2021Article
- Compartmentalization and metabolic regulation of glycolysis.Journal of cell science · 2021Review
- What fuels the fly: Energy metabolism inScience advances · 2021Review
- CytochromeCells · 2021Review
- Metabolism and growth adaptation to environmental conditions in Drosophila.Cellular and molecular life sciences : CMLS · 2020Review
- What is the rate-limiting step towards aging? Chemical reaction kinetics might reconcile contradictory observations in experimental aging research.GeroScience · 2020Article
- The Role of Muscle in Insect Energy Homeostasis.Frontiers in physiology · 2020Review
- Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness.PLoS genetics · 2018Review
- Organ reserve, excess metabolic capacity, and aging.Biogerontology · 2018Review
- Regulation of Carbohydrate Energy Metabolism inGenetics · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
An important question in evolutionary and physiological genetics is how the control of flux-base phenotypes is distributed across the enzymes in a pathway. This control is often related to enzyme-specific levels of activity that are reported to be in excess of that required for demand. In glycolysis, metabolic control is frequently considered vested in classical regulatory enzymes, each strongly displaced from equilibrium. Yet the contribution of individual steps to control is unclear. To assess enzyme-specific control in the glycolytic pathway, we used P-element excision-derived mutagenesis in Drosophila melanogaster to generate full and partial knockouts of seven metabolic genes and to measure tethered flight performance. For most enzymes, we find that reduction to half of the normal activity has no measurable impact on wing beat frequency. The enzymes catalyzing near-equilibrium reactions, phosphoglucose isomerase, phosphoglucomutase, and triosephosphate isomerase fail to show any decline in flight performance even when activity levels are reduced to 17% or less. At reduced activities, the classic regulatory enzymes, hexokinase and glycogen phosphorylase, show significant drops in flight performance and are nearer to saturation. Our results show that flight performance is canalized or robust to the activity variation found in natural populations. Furthermore, enzymes catalyzing near-equilibrium reactions show strong genetic dominance down to low levels of activity. This implies considerable excess enzyme capacity for these enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.