ArticleInfection and immunity2007
Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 139 citations in OpenAlex.
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- Culturing of a complex gut microbial community in mucin-hydrogel carriers reveals strain- and gene-associated spatial organization.Nature communications · 2023Article
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- A genetic regulatory see-saw of biofilm and virulence in MRSA pathogenesis.Frontiers in microbiology · 2023Review
- A Review of Biofilm Formation ofAntibiotics (Basel, Switzerland) · 2022Review
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- Interplay of CodY and CcpA in Regulating Central Metabolism and Biofilm Formation in Staphylococcus aureus.Journal of bacteriology · 2022Article
- Efflux pump inhibitors: new updates.Pharmacological reports : PR · 2021Review
- Small-Molecule Compound SYG-180-2-2 to Effectively Prevent the Biofilm Formation of Methicillin-ResistantFrontiers in microbiology · 2021Article
- An Overview of Biological and Computational Methods for Designing Mechanism-Informed Anti-biofilm Agents.Frontiers in microbiology · 2021Review
- Effects of Tigecycline Combined with Azithromycin Against Biofilms of Multidrug-ResistantInfection and drug resistance · 2021Article
- Mfd Affects Global Transcription and the Physiology of StressedFrontiers in microbiology · 2021Article
- Staphylococcal Biofilm Development: Structure, Regulation, and Treatment Strategies.Microbiology and molecular biology reviews : MMBR · 2020Review
- The DEAD-box RNA helicase CshA is required for fatty acid homeostasis in Staphylococcus aureus.PLoS genetics · 2020Article
- The Impacts ofInfection and immunity · 2020Article
- Genetic screens reveal novel major and minor players in magnesium homeostasis of Staphylococcus aureus.PLoS genetics · 2019Article
- Regulation ofMicrobiology spectrum · 2019Review
- Mfd protects against oxidative stress in Bacillus subtilis independently of its canonical function in DNA repair.BMC microbiology · 2019Article
19 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus produces biofilm and this mode of colonization facilitates infections that are often difficult to treat and engender high morbidity and mortality. We have exploited bacteriophage Mu transposition methods to create an insertional mutant library in a highly biofilm-forming S. aureus clinical isolate. Our screen identified 38 insertions in 23 distinct genes together with one intergenic region that significantly reduced biofilm formation. Nineteen insertions were mapped in loci not previously known to affect biofilm in this organism. These include insertions in codY, srrA, mgrA, and fmtA, a putative DEAD-box helicase, two members of the zinc-metallo-beta lactamase/beta-CASP family, and a hypothetical protein with a GGDEF motif. Fifteen insertions occurred in the icaADBC operon, which produces intercellular adhesion antigen (PIA) and is important for biofilm formation in many strains of S. aureus and Staphylococcus epidermidis. Obtaining a high proportion of independent Em-Mu disruptions in icaADBC demonstrated both the importance of PIA for biofilm formation in this clinical strain and the strong validation of the screening procedure that concomitantly uncovered additional mutants. All non-ica mutants were further analyzed by immunoblotting and biochemical fractionation for perturbation of PIA and wall teichoic acid. PIA levels were diminished in the majority of non-ica insertional mutants. Three mutant strains were chosen and were functionally complemented for restored biofilm formation by transformation with plasmids carrying the cloned wild-type gene under the control of a xylose-inducible promoter. This is a comprehensive collection of biofilm-defective mutants that underscores the multifactorial genetic program underlying the establishment of biofilm in this insidious pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.