ArticleInfection and immunity2007
Enterohemorrhagic Escherichia coli O157:H7 gal mutants are sensitive to bacteriophage P1 and defective in intestinal colonization.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
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- Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.Microbial cell factories · 2024Article
- Bacterial Virus Forcing of Bacterial O-Antigen Shields: Lessons from Coliphages.International journal of molecular sciences · 2023Review
- O antigen biogenesis sensitises Escherichia coli K-12 to bile salts, providing a plausible explanation for its evolutionary loss.PLoS genetics · 2023Article
- Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into Multiple Gut Pathogens.ACS synthetic biology · 2023Article
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- Phase separation in the outer membrane ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Genome-Wide Analyses of the Temperature-Responsive Genetic Loci of the Pectinolytic Plant PathogenicInternational journal of molecular sciences · 2021Article
- High-throughput mapping of the phage resistance landscape in E. coli.PLoS biology · 2020Article
- Structure and function of bacteriophage CBA120 ORF211 (TSP2), the determinant of phage specificity towards E. coli O157:H7.Scientific reports · 2020Article
- The role of O-polysaccharide chain and complement resistance of Escherichia coli in mammary virulence.Veterinary research · 2020Article
- Genome-wide screens reveal Escherichia coli genes required for growth of T1-like phage LL5 and V5-like phage LL12.Scientific reports · 2020Article
- Combating Actions of Green 2D-Materials on Gram Positive and Negative Bacteria and Enveloped Viruses.Frontiers in bioengineering and biotechnology · 2020Article
- Structure and tailspike glycosidase machinery of ORF212 from E. coli O157:H7 phage CBA120 (TSP3).Scientific reports · 2019Article
- The influence of surface chemistry on the kinetics and thermodynamics of bacterial adhesion.Scientific reports · 2018Article
- Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes.Journal of visualized experiments : JoVE · 2018Article
- Influence of RNase E deficiency on the production of stx2-bearing phages and Shiga toxin in an RNase E-inducible strain of enterohaemorrhagic Escherichia coli (EHEC) O157:H7.Journal of medical microbiology · 2018Article
- Effective Pro-Inflammatory Induced Activity of GALT, a Conserved Antigen inFrontiers in cellular and infection microbiology · 2018Article
- Screening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase.Journal of enzyme inhibition and medicinal chemistry · 2017Article
- Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7.FEMS microbiology letters · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli (EHEC), especially E. coli O157:H7, is an emerging cause of food-borne illness. Unfortunately, E. coli O157 cannot be genetically manipulated using the generalized transducing phage P1, presumably because its extensive O antigen obscures the P1 receptor, the lipopolysaccharide (LPS) core subunit. The GalE, GalT, GalK, and GalU proteins are necessary for modifying galactose before it can be assembled into the repeating subunit of the O antigen. Here, we constructed E. coli O157:H7 gal mutants which presumably have little or no O antigen. These strains were able to adsorb P1. P1 lysates grown on the gal mutant strains could be used to move chromosomal markers between EHEC strains, thereby facilitating genetic manipulation of E. coli O157:H7. The gal mutants could easily be reverted to a wild-type Gal(+) strain using P1 transduction. We found that the O157:H7 galETKM::aad-7 deletion strain was 500-fold less able to colonize the infant rabbit intestine than the isogenic Gal(+) parent, although it displayed no growth defect in vitro. Furthermore, in vivo a Gal(+) revertant of this mutant outcompeted the galETKM deletion strain to an extent similar to that of the wild type. This suggests that the O157 O antigen is an important intestinal colonization factor. Compared to the wild type, EHEC gal mutants were 100-fold more sensitive to a peptide derived from bactericidal permeability-increasing protein, a bactericidal protein found on the surface of intestinal epithelial cells. Thus, one way in which the O157 O antigen may contribute to EHEC intestinal colonization is to promote resistance to host-derived antimicrobial polypeptides.
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