ArticleEukaryotic cell2006
Proteins of the glycine decarboxylase complex in the hydrogenosome of Trichomonas vaginalis.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Extreme mitochondrial reduction in a novel group of free-living metamonads.Nature communications · 2024Article
- Hydrogenosome, Pairing Anaerobic Fungi and HJournal of fungi (Basel, Switzerland) · 2022Review
- Organelles that illuminate the origins ofNature ecology & evolution · 2017Article
- Hydrogenosomes in the diplomonad Spironucleus salmonicida.Nature communications · 2013Article
- Article
- The Trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.International journal for parasitology · 2011Article
- The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis.PloS one · 2011Article
- Sawyeria marylandensis (Heterolobosea) has a hydrogenosome with novel metabolic properties.Eukaryotic cell · 2010Article
- Lipoic acid metabolism in microbial pathogens.Microbiology and molecular biology reviews : MMBR · 2010Review
- Diversity and reductive evolution of mitochondria among microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Mitochondrion-related organelles in eukaryotic protists.Annual review of microbiology · 2010Review
- Genetic evidence for a mitochondriate ancestry in the 'amitochondriate' flagellate Trimastix pyriformis.PloS one · 2008Article
- Identification and biochemical characterization of serine hydroxymethyl transferase in the hydrogenosome of Trichomonas vaginalis.Eukaryotic cell · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Trichomonas vaginalis is a unicellular eukaryote that lacks mitochondria and contains a specialized organelle, the hydrogenosome, involved in carbohydrate metabolism and iron-sulfur cluster assembly. We report the identification of two glycine cleavage H proteins and a dihydrolipoamide dehydrogenase (L protein) of the glycine decarboxylase complex in T. vaginalis with predicted N-terminal hydrogenosomal presequences. Immunofluorescence analyses reveal that both H and L proteins are localized in hydrogenosomes, providing the first evidence for amino acid metabolism in this organelle. All three proteins were expressed in Escherichia coli and purified to homogeneity. The experimental Km of L protein for the two H proteins were 2.6 microM and 3.7 microM, consistent with both H proteins serving as substrates of L protein. Analyses using purified hydrogenosomes showed that endogenous H proteins exist as monomers and endogenous L protein as a homodimer in their native states. Phylogenetic analyses of L proteins revealed that the T. vaginalis homologue shares a common ancestry with dihydrolipoamide dehydrogenases from the firmicute bacteria, indicating its acquisition via a horizontal gene transfer event independent of the origins of mitochondria and hydrogenosomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.