ArticleEukaryotic cell2007
Small-subunit rRNA processome proteins are translationally regulated during differentiation of Trypanosoma cruzi.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Metacyclogenesis as the Starting Point of Chagas Disease.International journal of molecular sciences · 2023Review
- Disruption of the inositol phosphorylceramide synthase gene affects Trypanosoma cruzi differentiation and infection capacity.PLoS neglected tropical diseases · 2023Article
- Signaling pathways involved in environmental sensing in Trypanosoma cruzi.Molecular microbiology · 2021Review
- Article
- Transcriptomic changes across the life cycle ofPeerJ · 2020Article
- Analysis of the In Vivo Translation Process in Trypanosoma cruzi Using Ribosome Profiling.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.BMC genomics · 2015Article
- Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform.PLoS neglected tropical diseases · 2013Article
- Origin and evolution of the eukaryotic SSU processome revealed by a comprehensive genomic analysis and implications for the origin of the nucleolus.Genome biology and evolution · 2013Article
- mRNA localization mechanisms in Trypanosoma cruzi.PloS one · 2013Article
- A 43-nucleotide U-rich element in 3'-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA abundance in intracellular amastigotes.The Journal of biological chemistry · 2012Article
- TbENF is an essential TbTFIIB-interacting trypanosomatid-specific factor.Molecular and biochemical parasitology · 2012Article
- Gene expression in trypanosomatid parasites.Journal of biomedicine & biotechnology · 2010Review
- The steady-state transcriptome of the four major life-cycle stages of Trypanosoma cruzi.BMC genomics · 2009Article
- The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis.Molecular microbiology · 2008Article
- Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruzi.The Journal of biological chemistry · 2008Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used differential display to select genes differentially expressed during differentiation of epimastigotes into metacyclic trypomastigotes in the protozoan parasite Trypanosoma cruzi. One of the selected clones had a sequence similar to that of the small-subunit (SSU) processome protein Sof1p, which is involved in rRNA processing. The corresponding T. cruzi protein, TcSof1, displayed a nuclear localization and is downregulated during metacyclogenesis. Heterologous RNA interference assays showed that depletion of this protein impaired growth but did not affect progression through the cell cycle, suggesting that ribosome synthesis regulation and the cell cycle are uncoupled in this parasite. Quantitative PCR (qPCR) assays of several SSU processome-specific genes in T. cruzi also showed that most of them were regulated posttranscriptionally. This process involves the accumulation of mRNA in the polysome fraction of metacyclic trypomastigotes, where TcSof1 cannot be detected. Metacyclic trypomastigote polysomes were purified and separated by sucrose gradient sedimentation. Northern blot analysis of the sucrose gradient fractions showed the association of TcSof1 mRNA with polysomes, confirming the qPCR data. The results suggest that the mechanism of regulation involves the blocking of translation elongation and/or termination.
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