ArticleJournal of bacteriology2007
Localization of the naturally occurring plasmid ColE1 at the cell pole.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- ProFiT-SPEci-FISH: a novel approach for linking plasmids to hosts in complex microbial communities at the single-cell level.Microbiome · 2025Article
- Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states.Biochemistry and biophysics reports · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Article
- PlasmidFrontiers in molecular biosciences · 2022Article
- Single-cell measurement of plasmid copy number and promoter activity.Nature communications · 2021Article
- Reproducible Antigen Recognition by the Type I-F CRISPR-Cas System.The CRISPR journal · 2020Article
- Effects of spatial heterogeneity on bacterial genetic circuits.PLoS computational biology · 2020Article
- Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance.Nature communications · 2019Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- High-Copy-Number Plasmid Segregation-Single-Molecule Dynamics in Single Cells.Biophysical journal · 2019Article
- Genetic control of ColE1 plasmid stability that is independent of plasmid copy number regulation.Current genetics · 2019Article
- SmallFrontiers in microbiology · 2019Article
- Brownian Ratchet Mechanism for Faithful Segregation of Low-Copy-Number Plasmids.Biophysical journal · 2017Article
- Plasmid stability analysis based on a new theoretical model employing stochastic simulations.PloS one · 2017Article
- Quantitative Localization Microscopy Reveals a Novel Organization of a High-Copy Number Plasmid.Biophysical journal · 2016Article
- Localization of Low Copy Number Plasmid pRC4 in Replicating Rod and Non-Replicating Cocci Cells of Rhodococcus erythropolis PR4.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The naturally occurring plasmid ColE1 was found to localize as a cluster in one or both of the cell poles of Escherichia coli. In addition to the polar localization of ColE1 in most cells, movement of the plasmid to the midcell position was observed in time-lapse studies. ColE1 could be displaced from its polar location by the p15A replicon, pBAD33, but not by plasmid RK2. The displacement of ColE1 by pBAD33 resulted in an almost random positioning of ColE1 foci in the cell and also in a loss of segregational stability, as evidenced by the large number of cells carrying pBAD33 with no visible ColE1 focus and as confirmed by ColE1 stability studies. The addition of the active partitioning systems of the F plasmid (sopABC) or RK2 (O(B1) incC korB) resulted in movement of the ColE1 replicon from the cell pole to within the nucleoid region. This repositioning did not result in destabilization but did result in an increase in the number of plasmid foci, most likely due to partial declustering. These results are consistent with the importance of par regions to the localization of plasmids to specific regions of the cell and demonstrate both localization and dynamic movement for a naturally occurring plasmid that does not encode a replication initiation protein or a partitioning system that is required for plasmid stability.
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Registered trials
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