ArticleApplied and environmental microbiology2007
Isolation and characterization of brewer's yeast variants with improved fermentation performance under high-gravity conditions.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Comparative assessment of food-grade osmolytes for enhancing yeast fermentation performance under salt stress.Microbiology spectrum · 2026Article
- Beer yeast breeding in the era of innovation: advances and applications for modern brewing.Biotechnology letters · 2025Review
- Obtaining new brewing yeasts using regional Chilean wine yeasts through an adaptive evolution program.Frontiers in microbiology · 2025Article
- Transcriptomic and metabolomic analysis reveals genes related to stress tolerance in high gravity brewing.World journal of microbiology & biotechnology · 2022Article
- Packing a punch: understanding how flavours are produced in lager fermentations.FEMS yeast research · 2021Review
- Lager-brewing yeasts in the era of modern genetics.FEMS yeast research · 2019Review
- Diacetyl control during brewery fermentation via adaptive laboratory engineering of the lager yeast Saccharomyces pastorianus.Journal of industrial microbiology & biotechnology · 2018Article
- Enhanced Wort Fermentation withApplied and environmental microbiology · 2018Article
- Novel brewing yeast hybrids: creation and application.Applied microbiology and biotechnology · 2017Review
- Evolutionary Engineering in Chemostat Cultures for Improved Maltotriose Fermentation Kinetics inFrontiers in microbiology · 2017Article
- Ploidy influences the functional attributes of de novo lager yeast hybrids.Applied microbiology and biotechnology · 2016Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- The microbiology of malting and brewing.Microbiology and molecular biology reviews : MMBR · 2013Review
- Chimeric protein complexes in hybrid species generate novel phenotypes.PLoS genetics · 2013Article
- Very high gravity (VHG) ethanolic brewing and fermentation: a research update.Journal of industrial microbiology & biotechnology · 2011Review
- Selection from industrial lager yeast strains of variants with improved fermentation performance in very-high-gravity worts.Applied and environmental microbiology · 2010Article
- Progress in metabolic engineering of Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2008Review
- Lager yeasts possess dynamic genomes that undergo rearrangements and gene amplification in response to stress.Current genetics · 2008Article
- Enhanced ethanol production at commercial scale from molasses using high gravity technology by mutant S. cerevisiae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
- Xylose isomerase improves growth and ethanol production rates from biomass sugars for both Saccharomyces pastorianus and Saccharomyces cerevisiae.Biotechnology progressArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To save energy, space, and time, today's breweries make use of high-gravity brewing in which concentrated medium (wort) is fermented, resulting in a product with higher ethanol content. After fermentation, the product is diluted to obtain beer with the desired alcohol content. While economically desirable, the use of wort with an even higher sugar concentration is limited by the inability of brewer's yeast (Saccharomyces pastorianus) to efficiently ferment such concentrated medium. Here, we describe a successful strategy to obtain yeast variants with significantly improved fermentation capacity under high-gravity conditions. We isolated better-performing variants of the industrial lager strain CMBS33 by subjecting a pool of UV-induced variants to consecutive rounds of fermentation in very-high-gravity wort (>22 degrees Plato). Two variants (GT336 and GT344) showing faster fermentation rates and/or more-complete attenuation as well as improved viability under high ethanol conditions were identified. The variants displayed the same advantages in a pilot-scale stirred fermenter under high-gravity conditions at 11 degrees C. Microarray analysis identified several genes whose altered expression may be responsible for the superior performance of the variants. The role of some of these candidate genes was confirmed by genetic transformation. Our study shows that proper selection conditions allow the isolation of variants of commercial brewer's yeast with superior fermentation characteristics. Moreover, it is the first study to identify genes that affect fermentation performance under high-gravity conditions. The results are of interest to the beer and bioethanol industries, where the use of more-concentrated medium is economically advantageous.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.