ArticleBiophysical journal2007
Quantitative membrane electrostatics with the atomic force microscope.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Discriminating Between Living and Dead Bacteria With a Field-Effect Transistor Based on Nano-Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Atomic force microscopy as an advanced tool in neuroscience.Translational neuroscience · 2015Review
- Ionizable Nitroxides for Studying Local Electrostatic Properties of Lipid Bilayers and Protein Systems by EPR.Methods in enzymology · 2015Article
- Neuron biomechanics probed by atomic force microscopy.International journal of molecular sciences · 2013Review
- Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy.Biophysical journal · 2013Article
- Surface electrostatics of lipid bilayers by EPR of a pH-sensitive spin-labeled lipid.Biophysical journal · 2013Article
- Changes in plasma membrane surface potential of PC12 cells as measured by Kelvin probe force microscopy.PloS one · 2012Article
- Probing the lipid membrane dipole potential by atomic force microscopy.Biophysical journal · 2008Article
- Test of the Gouy-Chapman theory for a charged lipid membrane against explicit-solvent molecular dynamics simulations.Physical review letters · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The atomic force microscope (AFM) is sensitive to electric double layer interactions in electrolyte solutions, but provides only a qualitative view of interfacial electrostatics. We have fully characterized silicon nitride probe tips and other experimental parameters to allow a quantitative electrostatic analysis by AFM, and we have tested the validity of a simple analytical force expression through numerical simulations. As a test sample, we have measured the effective surface charge density of supported zwitterionic dioleoylphosphatidylcholine membranes with a variable fraction of anionic dioleoylphosphatidylserine. The resulting surface charge density and surface potential values are in quantitative agreement with those predicted by the Gouy-Chapman-Stern model of membrane charge regulation, but only when the numerical analysis is employed. In addition, we demonstrate that the AFM can detect double layer forces at a separation of several screening lengths, and that the probe only perturbs the membrane surface potential by <2%. Finally, we demonstrate 50-nm resolution electrostatic mapping on heterogeneous model membranes with the AFM. This novel combination of capabilities demonstrates that the AFM is a unique and powerful probe of membrane electrostatics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.