ArticleThe Journal of physiology2007
Water transport by GLUT2 expressed in Xenopus laevis oocytes.
Article in The Journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- A budget for brain metabolic water production by glucose catabolism during rest, rises in activity and sleep.Fluids and barriers of the CNS · 2025Review
- Structural Dynamics of Neutral Amino Acid Transporter SLC6A19 in Simple and Complex Lipid Bilayers.Journal of cellular biochemistry · 2025Article
- Toward New AQP4 Inhibitors: ORI-TRN-002.International journal of molecular sciences · 2024Article
- Acute effects of empagliflozin on open-loop baroreflex function and urinary glucose excretion in rats with chronic myocardial infarction.The journal of physiological sciences : JPS · 2023Article
- Acute effects of empagliflozin on open-loop baroreflex function and urine glucose excretion in Goto-Kakizaki diabetic rats.The journal of physiological sciences : JPS · 2023Article
- Non-Aquaporin Water Channels.Advances in experimental medicine and biology · 2023Article
- Molecular mechanisms of brain water transport.Nature reviews. Neuroscience · 2021Review
- Computational Modelling of Glucose Uptake by SGLT1 and Apical GLUT2 in the Enterocyte.Frontiers in physiology · 2021Article
- Rhinella marina oocytes: a suitable alternative expression system for functional characterization of aquaglyceroporins.Scientific reports · 2019Article
- A de novo Ser111Thr variant in aquaporin-4 in a patient with intellectual disability, transient signs of brain ischemia, transient cardiac hypertrophy, and progressive gait disturbance.Cold Spring Harbor molecular case studies · 2018Article
- Structural and functional significance of water permeation through cotransporters.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Molecular pathophysiology of cerebral edema.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2016Review
- Transepithelial glucose transport and Na+/K+ homeostasis in enterocytes: an integrative model.American journal of physiology. Cell physiology · 2014Article
- Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE.Nature communications · 2014Article
- Aquaporins: important but elusive drug targets.Nature reviews. Drug discovery · 2014Review
- Does apical membrane GLUT2 have a role in intestinal glucose uptake?F1000Research · 2014Review
- Transmembrane water-flux through SLC4A11: a route defective in genetic corneal diseases.Human molecular genetics · 2013Article
- Transient formation of water-conducting states in membrane transporters.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The role of aquaporin and tight junction proteins in the regulation of water movement in larval zebrafish (Danio rerio).PloS one · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The glucose transporter GLUT2 has been shown to also transport water. In the present paper we investigated the relation between sugar and water transport in human GLUT2 expressed in Xenopus oocytes. Sugar transport was determined from uptakes of non-metabolizable glucose analogues, primarily 3-O-methyl-D-glucopyranoside; key experimental results were confirmed using D(+)-glucose. Water transport was derived from changes in oocyte volume monitored at a high resolution (20 pl, 1 s). Expression of GLUT2 induced a sugar permeability, P(S), of about 5 x 10(-6) cm s(-1) and a passive water permeability, L(p), of 5.5 x 10(-5) cm s(-1). Accordingly, the passive water permeability of a GLUT2 protein is about 10 times higher than its sugar permeability. Both permeabilities were abolished by phloretin. Isosmotic addition of sugar to the bathing solution (replacing mannitol) induced two parallel components of water influx in GLUT2, one by osmosis and one by cotransport. The osmotic driving force arose from sugar accumulation at the intracellular side of the membrane and was given by an intracellular diffusion coefficient for sugar of 10(-6) cm(2) s(-1), one-fifth of the free solution value. The diffusion coefficient was determined in oocytes coexpressing GLUT2 and the water channel AQP1 where water transport was predominantly osmotic. By the cotransport mechanism about 35 water molecules were transported for each sugar molecule by a mechanism within the GLUT2. These water molecules could be transported uphill, against an osmotic gradient, energized by the flux of sugar. This capacity for cotransport is 10 times smaller than that of the Na(+)-coupled glucose transporters (SGLT1). The physiological role of GLUT2 for intestinal transport under conditions of high luminal sugar concentrations is discussed.
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