ArticleNucleic acids research2007
Dispersion of the RmInt1 group II intron in the Sinorhizobium meliloti genome upon acquisition by conjugative transfer.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome.Frontiers in molecular biosciences · 2022Article
- Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.FEMS microbiology reviews · 2021Review
- Synthetase of the methyl donor S-adenosylmethionine from nitrogen-fixing α-rhizobia can bind functionally diverse RNA species.RNA biology · 2021Article
- Article
- Bacterial group II introns generate genetic diversity by circularization and trans-splicing from a population of intron-invaded mRNAs.PLoS genetics · 2018Article
- Contribution of Mobile Group II Introns toFrontiers in microbiology · 2018Review
- Recent horizontal transfer, functional adaptation and dissemination of a bacterial group II intron.BMC evolutionary biology · 2016Article
- Circularization pathway of a bacterial group II intron.Nucleic acids research · 2016Article
- Functionality ofFrontiers in molecular biosciences · 2016Article
- Article
- Localization of a bacterial group II intron-encoded protein in human cells.Scientific reports · 2015Article
- Inactivation of group II intron RmInt1 in the Sinorhizobium meliloti genome.Scientific reports · 2015Article
- Complete Genome Sequence of the RmInt1 Group II Intronless Sinorhizobium meliloti Strain RMO17.Genome announcements · 2014Article
- Article
- Insights into the strategies used by related group II introns to adapt successfully for the colonisation of a bacterial genome.RNA biology · 2014Review
- Use of the computer-retargeted group II intron RmInt1 of Sinorhizobium meliloti for gene targeting.RNA biology · 2014Article
- Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.PloS one · 2013Article
- Article
- Relevance of the branch point adenosine, coordination loop, and 3' exon binding site for in vivo excision of the Sinorhizobium meliloti group II intron RmInt1.The Journal of biological chemistry · 2011Article
- Spread of the group II intron RmInt1 and its insertion sequence target sites in the plant endosymbiont Sinorhizobium meliloti.Mobile genetic elements · 2011Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
RmInt1 is a self-splicing and mobile group II intron initially identified in the bacterium Sinorhizobium meliloti, which encodes a reverse transcriptase-maturase (Intron Encoded Protein, IEP) lacking the C-terminal DNA binding (D) and DNA endonuclease domains (En). RmInt1 invades cognate intronless homing sites (ISRm2011-2) by a mechanism known as retrohoming. This work describes how the RmInt1 intron spreads in the S.meliloti genome upon acquisition by conjugation. This process was revealed by using the wild-type intron RmInt1 and engineered intron-donor constructs based on ribozyme coding sequence (DeltaORF)-derivatives with higher homing efficiency than the wild-type intron. The data demonstrate that RmInt1 propagates into the S.meliloti genome primarily by retrohoming with a strand bias related to replication of the chromosome and symbiotic megaplasmids. Moreover, we show that when expressed in trans from a separate plasmid, the IEP is able to mobilize genomic DeltaORF ribozymes that afterward displayed wild-type levels of retrohoming. Our results contribute to get further understanding of how group II introns spread into bacterial genomes in nature.
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