Evidence map›Paper›PMID 17157717›Full record

ArticleAlcohol (Fayetteville, N.Y.)2006

Microarray analysis identifies cerebellar genes sensitive to chronic ethanol treatment in PKCgamma mice.

Barbara J Bowers, Richard A Radcliffe, Amy M Smith, Jill Miyamoto-Ditmon, Jeanne M Wehner

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Alcohol (Fayetteville, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

  1. Potassium Channels and Their Potential Roles in Substance Use Disorders.International journal of molecular sciences · 2021
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review. Neurogenetic studies of alcohol addiction.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008
    Review
  10. Review
  11. Communication networks in the brain: neurons, receptors, neurotransmitters, and alcohol.Alcohol research & health : the journal of the National Institute on Alcohol Abuse and Alcoholism · 2008
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Barbara J BowersInstitute for Behavioral Genetics, University of Colorado, 447 UCB, Boulder, CO 80309, USA. bbowers@colorado.edu
Richard A Radcliffe
Amy M Smith
Jill Miyamoto-Ditmon
Jeanne M Wehner
University of Colorado Boulder · USUniversity of Colorado Health · US

Funding

TRANSGENIC MOUSE MODELS TO STUDY THE ROLE OF NEUROTENSIN RECEPTORP50AA003527 · NIAAA · UNIVERSITY OF COLORADO DENVER · PI DEITRICH, RICHARD A · 1985 to 2003
$5.9M
Role of Nicotinic Receptors in Effects of AlcoholR01AA013018 · NIAAA · UNIVERSITY OF COLORADO AT BOULDER · PI WEHNER, JEANNE M · 2002 to 2006
$2.2M
Gene Expression and Drug-Induced Sensitivity to AlcoholR01AA013177 · NIAAA · UNIVERSITY OF COLORADO DENVER · PI RADCLIFFE, RICHARD A · 2002 to 2004
$915k
5-HT2 and 5-HT1A Receptors in PKC-gamma Null Mutant MiceR21AA013901 · NIAAA · UNIVERSITY OF COLORADO AT BOULDER · PI BOWERS, BARBARA J · 2003 to 2005
$446k
QTL MAPPING OF COMPLEX LEARNING IN MICER01MH053668 · NIMH · UNIVERSITY OF COLORADO AT BOULDER · PI WEHNER, JEANNE M · 1995 to 2001
$355k
NIAAA NIH HHS AA03527NIAAA NIH HHS AA13018NIAAA NIH HHS AA13177NIAAA NIH HHS AA13901NIAAA NIH HHS R01 AA013018NIAAA NIH HHS R01 AA013177NIAAA NIH HHS R21 AA013901NIMH NIH HHS MH53668
6 · The paper itself

Abstract

Neuroadaptive changes that occur in the development of ethanol tolerance may be the result of alterations in gene expression. We have shown that PKCgamma wild-type mice develop tolerance to the sedative-hypnotic effects of ethanol after chronic ethanol treatment; whereas, mutant mice do not, making these genotypes a suitable model for identifying changes in gene expression related to tolerance development. Using a two-stage process, several genes were initially identified using microarray analyses of cerebellar tissue from ethanol-treated PKCgamma mutant and wild-type mice. Subsequent confirmation of a subset of these genes using quantitative real time reverse transcriptase polymerase chain reactions (qRT-PCR) was done to verify gene expression changes. A total of 109 genes from different functional classifications were identified in these groups on the microarrays. Eight genes were selected for verification as follows: three, Twik-1, Plp, and Adk2, were chosen as genes related to tolerance; another three, Hsp70.2, Bdnf, and Th, were chosen as genes related to resistance to tolerance; and two genes, JunB and Nur77, were selected as candidate genes sensitive to chronic ethanol. The results from the verification experiments indicated that Twik-1, which codes for a potassium channel, was associated with tolerance and appeared to be dependent on the presence of PKCgamma. No genes were confirmed to be related to resistance to tolerance; however, expression of two of these, Hsp70.2 and Th, were found to be sensitive to chronic ethanol and were added to the transcription factors, JunB and Nur77, confirmed by qRT-PCR, as a subset of genes that respond to chronic ethanol.

Indexed as

Gene Expression ProfilingOligonucleotide Array Sequence AnalysisAlcoholismAnimalsCentral Nervous System DepressantsCerebellumDNA-Binding ProteinsDrug ToleranceEthanolGene Expression RegulationHSP70 Heat-Shock ProteinsMiceMice, Inbred C57BLMice, KnockoutMyelin Proteolipid ProteinNerve Tissue ProteinsCentral Nervous System DepressantsDNA-Binding ProteinsEthanolHSP70 Heat-Shock ProteinsHspa2 protein, mouseKcnk1 protein, mouseMyelin Proteolipid ProteinNerve Tissue ProteinsNr4a1 protein, mouseNuclear Receptor Subfamily 4, Group A, Member 1Plp1 protein, mousePotassium Channels, Tandem Pore DomainProtein Kinase Cprotein kinase C gammaProto-Oncogene Proteins c-junReceptors, Cytoplasmic and NuclearReceptors, SteroidRNA, MessengerTranscription FactorsTyrosine 3-Monooxygenase

Identifiers

PMID17157717
PMCPMC1931504
OpenAlexW2050506157

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.