ArticleBiochimica et biophysica acta2007
Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA.
Article in Biochimica et biophysica acta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Looking Back, Moving Forward: Lipid Nanoparticles as a Promising Frontier in Gene Delivery.ACS pharmacology & translational science · 2023Review
- Review
- The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids.International journal of molecular sciences · 2021Article
- Preparation of cationic proteoliposomes using cell-free membrane protein synthesis: the chaperoning effect of cationic liposomes.RSC advances · 2020Article
- Effect of white mange mixture in a murine model of psoriasis.Experimental and therapeutic medicine · 2019Article
- Endosomal Escape and Cytosolic Penetration of Macromolecules Mediated by Synthetic Delivery Agents.Bioconjugate chemistry · 2019Review
- Morphological Analysis of Trafficking and Processing of Anionic and Cationic Liposomes in Cultured Cells.Acta histochemica et cytochemica · 2018Article
- Article
- Highly efficient cationic ethylphosphatidylcholine siRNA carrier for GFP suppression in modified breast cancer cells.Anticancer research · 2012Article
- Article
- Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency.International journal of nanomedicine · 2012Article
- Probing the in vitro mechanism of action of cationic lipid/DNA lipoplexes at a nanometric scale.Nucleic acids research · 2011Article
- Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Natural lipid extracts and biomembrane-mimicking lipid compositions are disposed to form nonlamellar phases, and they release DNA from lipoplexes most efficiently.Biochimica et biophysica acta · 2007Article
- Synergy in lipofection by cationic lipid mixtures: superior activity at the gel-liquid crystalline phase transition.The journal of physical chemistry. B · 2007Article
- Hydrophobic moiety of cationic lipids strongly modulates their transfection activity.Molecular pharmaceuticsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Lipoplexes containing a mixture of cationic phospholipids dioleoylethylphosphatidylcholine (EDOPC) and dilauroylethylphosphatidylcholine (EDLPC) are known to be far more efficient agents in transfection of cultured primary endothelial cells than are lipoplexes containing either lipid alone. The large magnitude of the synergy permits comparison of the physical and physico-chemical properties of lipoplexes that have very different transfection efficiencies, but minor chemical differences. Here we report that the superior transfection efficiency of the EDLPC/EDOPC lipoplexes correlates with higher surface activity, higher affinity to interact and mix with negatively charged membrane-mimicking liposomes, and with considerably more efficient DNA release relative to the EDOPC lipoplexes. Observations on cultured cells agree with the results obtained with model systems; confocal microscopy of transfected human umbilical artery endothelial cells (HUAEC) demonstrated more extensive DNA release into the cytoplasm and nucleoplasm for the EDLPC/EDOPC lipoplexes than for EDOPC lipoplexes; electron microscopy of cells fixed and embedded directly on the culture dish revealed contact of EDLPC/EDOPC lipoplexes with various cellular membranes, including those of the endoplasmic reticulum, mitochondria and nucleus. The sequence of events outlining efficient lipofection is discussed based on the presented data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.