ArticleGenome biology2006
Generation of medaka gene knockout models by target-selected mutagenesis.
Article in Genome biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Genomic consequences of domestication and the diversification of body coloration and morphology in ornamental medaka strains.Molecular biology and evolution · 2026Article
- Article
- Evolutionary differentiation of androgen receptor is responsible for sexual characteristic development in a teleost fish.Nature communications · 2023Article
- Individual identification of inbred medaka based on characteristic melanophore spot patterns on the head.Scientific reports · 2023Article
- Article
- A rad50 germline mutation induces tumorigenesis and ataxia-telangiectasia phenotype in a transparent medaka model.PloS one · 2023Article
- Multiple isoforms of HSP70 and HSP90 required for betanodavirus multiplication in medaka cells.Archives of virology · 2022Article
- 3D reconstructed brain images reveal the possibility of the ogg1 gene to suppress the irradiation-induced apoptosis in embryonic brain in medaka (Oryzias latipes).Journal of radiation research · 2022Article
- Establishing an effective gene knockdown system using cultured cells of the model fish medaka (Biology methods & protocols · 2022Article
- Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops.Frontiers in genetics · 2022Article
- A point-mutation in the C-domain of CMP-sialic acid synthetase leads to lethality of medaka due to protein insolubility.Scientific reports · 2021Article
- Animal Models of Autosomal Recessive Parkinsonism.Biomedicines · 2021Review
- αβ/γδ T cell lineage outcome is regulated by intrathymic cell localization and environmental signals.Science advances · 2021Article
- Intact in vivo visualization of telencephalic microvasculature in medaka using optical coherence tomography.Scientific reports · 2020Article
- Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.Cell death and differentiation · 2020Article
- Sexually dimorphic role of oxytocin in medaka mate choice.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Involvement of Rev1 in alkylating agent-induced loss of heterozygosity in Oryzias latipes.Genes to cells : devoted to molecular & cellular mechanisms · 2020Article
- Renal lesions in leptin receptor-deficient medaka (Journal of toxicologic pathology · 2019Article
- Article
- Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool.BMC genomics · 2019Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.
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